cells

mast cell line

Cell Type:

Granulocyte

Tissue Origin:

Species:

human

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Efficiency	Viable Cells	Substrate		Format	Platform
L	T-020	40-50%	80-90%		µg	100 µl	I/II/2b
V	T-020	53%	76%	Plasmid (general)	1 µg	100 µl	I/II/2b
L	T-020	61%	93%	Plasmid (general)	1 µg	100 µl	I/II/2b
V	T-030	72%	100%	Plasmid (general)	1 µg	100 µl	I/II/2b
L	T-030	60%	100%	Plasmid (general)	1 µg	100 µl	I/II/2b

Citations

Annexin 1 cleavage in activated neutrophils: A pivotal role for proteinase 3

Categories:

Transfection

Authors:

Vong L, D'Acquisto F, Pederzoli-Ribeil M, Lavagno L, Flower RJ, Witko-Sarsat V, Perretti M

In:

J Biol Chem (2007) 282(41): 29998-30004

Progesterone reduces the migration of mast cells toward the chemokine stromal cell-derived factor-1/CXCL12 with an accompanying decrease in CXCR4 receptors

Categories:

Transfection

Authors:

Belot MP, Abdennebi-Najar L, Gaudin F, Lieberherr M, Godot V, Taieb J, Emilie D, Machelon V

In:

Am J Physiol Endocrinol Metab (2007) 292(5): E1410-7

HMC-1

Transfection Information

Citations