WI-38
Export
Lung embryonic fibroblasts line
Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SE
CP-104
5e5
65-69%
37-53%
Plasmid (general)
2 µg
100 µl
4D X-Unit
SE
CP-104
1e5
64-67%
48-52%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
R
V-001
5e5
66-84%
87-95%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
The Secretome of Human Bronchial Epithelial Cells Exposed to Fine Atmospheric Particles Induces Fibroblast Proliferation
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Boublil L, Martinon L, Baeza-Squiban A
In:
Other (2013) 4(2): 188-200
Definition of pRB- and p53-dependent and independent steps in HIRA/ASF1a-mediated formation of Senecence-Associated Heterochromatin Foci (SAHF)
Categories:
Transfection
Authors:
Ye X, Zerlanko B, Zhang R, Somaiah N, Lipinski M, Salomoni P, Adams PD
In:
Mol Cell Biol (2007) 27(7): 2452-65
HP1 proteins are essential for a dynamic nuclear response that rescues the function of perturbed heterochromatin in primary human cells
Categories:
Transfection
Authors:
Zhang R, Liu ST, Chen W, Bonner M, Pehrson J, Yen TJ, Adams PD
In:
Mol Cell Biol (2007) 27(3): 949-62
DNA end joining becomes less efficient and more error-prone during cellular senescence
Categories:
Transfection
Authors:
Seluanov A, Mittelman D, Pereira-Smith OM, Wilson JH and Gorbunova V
In:
Proc Natl Acad Sci USA (2004) 101(20): 7624-7629