WI-38

Lung embryonic fibroblasts line

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CP-104 5e5 65-69% 37-53% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE CP-104 1e5 64-67% 48-52% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
R V-001 5e5 66-84% 87-95% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Boublil L, Martinon L, Baeza-Squiban A 
In:
Other (2013) 4(2): 188-200 
Categories:
Transfection 
Authors:
Ye X, Zerlanko B, Zhang R, Somaiah N, Lipinski M, Salomoni P, Adams PD 
In:
Mol Cell Biol (2007) 27(7): 2452-65 
Categories:
Transfection 
Authors:
Zhang R, Liu ST, Chen W, Bonner M, Pehrson J, Yen TJ, Adams PD 
In:
Mol Cell Biol (2007) 27(3): 949-62 
Categories:
Transfection 
Authors:
Seluanov A, Mittelman D, Pereira-Smith OM, Wilson JH and Gorbunova V 
In:
Proc Natl Acad Sci USA (2004) 101(20): 7624-7629