HCT15
Export
Colon adenocarcinoma, epithelial
Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
O-017
1e6
74%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-016
1e6
80%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
U-020
1e6
78%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Phosphorylation of pRB at Ser612 by Chk1/2 leads to a complex between pRB and E2F-1 after DNA damage
Categories:
Transfection
Authors:
Inoue Y, Kitagawa M, Taya Y
In:
EMBO J (2007) 26(8): 2083-93