HCT15

Colon adenocarcinoma, epithelial

Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V O-017 1e6 74% 90% Plasmid (general) 2 µg 100 µl I/II/2b
V T-016 1e6 80% 90% Plasmid (general) 2 µg 100 µl I/II/2b
V U-020 1e6 78% 80% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Inoue Y, Kitagawa M, Taya Y 
In:
EMBO J (2007) 26(8): 2083-93