cells

B lymphoblast cell line isolated from Burkitt's lymphoma patient; EBV positive

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	L	A-020	1e6	51%	70%	Plasmid (general)	2 µg	100 µl	I/II/2b
	SF	CA-137	3e5			Plasmid (general)		20 µl	4D X-Unit

Citations

Allele-specific expression changes dynamically during T cell activation in HLA and other autoimmune loci

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Gutierrez-Arcelus, M., Baglaenko, Y., Arora, J., Hannes, S., Luo, Y., Amariuta, T., Teslovich, N., Rao, D. A., Ermann, J., Jonsson, A. H., NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium, Navarrete, C., Rich, S. S., Taylor, K. D., Rotter, J. I., Gregersen, P. K., Esko, T., Brenner, M. B., & Raychaudhuri, S.

In:

Nat Genet (2020) :

Selection of the alternative exon 1 from the cd5 gene down-regulates membrane level of the protein in B lymphocytes

Categories:

Transfection

Authors:

Garaud S, Le Dantec C, Berthou C, Lydyard PM, Youinou P, Renaudineau Y

In:

J Immunol (2008) 181(3): 2010-8

Gene expression analysis identifies novel RBL2/p130 target genes in endemic Burkitt's lymphoma cell lines and primary tumors

Categories:

Transfection

Authors:

De Falco G, Leucci E, Lenze D, Piccaluga PP, Claudio PP, Onnis A, Cerino G, Nyagol J, Mwanda W, Bellan C, Hummel M, Pileri S, Tosi P, Stein H, Giordano A, Leoncini L

In:

Blood (2007) 110(4): 1301-7

Talin1 Regulates TCR-Mediated LFA-1 Function

Categories:

Transfection

Authors:

Simonson WT, Franco SJ, Huttenlocher A

In:

J Immunol (2006) 177(11): 7707-14

Daudi

Transfection Information

Citations