cells

skin fibroblast

Cell Type:

Fibroblast

Tissue Origin:

dermal

Species:

equine

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	T	T-030	7e5	64%	90%	Plasmid (general)	5 µg	100 µl	I/II/2b
	T	U-023	7e5	57%	90%	Plasmid (general)	5 µg	100 µl	I/II/2b

Citations

The equine herpesvirus-1 IR3 gene that lies antisense to the sole immediate-early (IE) gene is trans-activated by the IE protein, and is poorly expressed to a protein

Categories:

Transfection

Authors:

Ahn BC, Breitenbach JE, Kim SK, O'Callaghan DJ

In:

Virology (2007) 363(1): 15-25

The Unique IR2 Protein of Equine Herpesvirus 1 Negatively Regulates Viral Gene Expression

Categories:

Transfection

Authors:

Kim SK, Ahn BC, Albrecht RA, O'callaghan DJ

In:

J Virol (2006) 80(10): 5041-9

NBL-6

Transfection Information

Citations