M-07e
Export
bcr-abl-deficient acute megakaryoblastic leukemia
Cell Type:
Megakaryocyte
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
S-011
4e6
30%
44%
Plasmid (general)
3 µg
100 µl
I/II/2b
R
S-002
4e6
29%
62%
Plasmid (general)
3 µg
100 µl
I/II/2b
L
T-020
2e6
40.9%
50%
Plasmid (general)
2 µg
100 µl
I/II/2b
L
X-001
2e6
61.7%
34%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
X-001
2e6
76.6%
42%
Plasmid (general)
2 µg
100 µl
I/II/2b
C
X-005
4e6
50%
60%
Plasmid (general)
2 µg
100 µl
I/II/2b
C
T-030
4e6
50%
60%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
c-Myb contributes to G2/M cell cycle transition in human hematopoietic cells by direct regulation of cyclin B1 expression
Categories:
Transfection
Authors:
Nakata Y, Shetzline S, Sakashita C, Kalota A, Rallapalli R, Rudnick SI, Zhang Y, Emerson SG, Gewirtz AM
In:
Mol Cell Biol (2007) 27(6): 2048-58
Integrin inhibition through Lyn-dependent cross talk from CXCR4 chemokine receptors in normal human CD34+ marrow cells
Categories:
Transfection
Authors:
Nakata Y, Tomkowicz B, Gewirtz AM, Ptasznik A
In:
Blood (2006) 107(11): 4234-9
Short interfering RNA (siRNA) targeting the Lyn kinase induces apoptosis in primary, and drug-resistant, BCR-ABL1(+) leukemia cells
Categories:
Transfection
Authors:
Ptasznik A, Nakata Y, Kalota A, Emerson SG and Gewirtz AM
In:
Nat Med (2004) 10(11): 1187-1189
Granulocyte-macrophage colony-stimulating factor and interleukin-3 induce cell cycle progression through the synthesis of c-Myc protein by internal ribosome entry site-mediated translation via phosphatidylinositol 3-kinase pathway in human factor-dependent leukemic cells
Categories:
Transfection
Authors:
Kobayashi N, Saeki K and Yuo A
In:
Blood (2003) 102(9): 3186-3195