cells

Murine mammary carcinoma cell line

Cell Type:

Mammary Epith.

Tissue Origin:

breast

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	T-016	1e6	60%	59%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-024	1e6	86%	51%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-027	1e6	64%	60%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	G-016	1e6	31%	78%	Plasmid (general)	2 µg	100 µl	I/II/2b
SE	CN-114				CRISPR/Cas		100 µl	4D X-Unit
SE	CM-150				CRISPR/Cas		20 µl	4D X-Unit
SE	CH-152						20 µl	4D X-Unit

Citations

SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms

Categories:

Transfection

Authors:

Ye Wang , Morvarid Mohseni , Angelo Grauel , Javier Estrada Diez , Wei Guan , Simon Liang , Jiyoung Elizabeth Choi , Minying Pu , Dongshu Chen , Tyler Laszewski , Stephanie Schwartz , Jane Gu , Leandra Mansur , Tyler Burks , Lauren Brodeur , Roberto Velazquez , Steve Kovats , Bhavesh Pant , Giri Buruzula , Emily Deng , Julie T Chen , Farid Sari-Sarraf , Christina Dornelas , Malini Varadarajan 1, Haiyan Yu , Chen Liu , Joanne Lim , Huai-Xiang Hao , Xiaomo Jiang , Anthony Malamas , Matthew J LaMarche , Felipe Correa Geyer , Margaret McLaughlin , Carlotta Costa , Joel Wagner , David Ruddy , Pushpa Jayaraman , Nathaniel Kirkpatrick , Pu Zhang , Oleg Iartchouk , Kimberly Aardalen , Viviana Cremasco , Glenn Dranoff , Jeffrey A Engelman , Serena Silver , Hongyun Wang, William D Hastings , Silvia Goldoni

In:

Scientific Reports (2021) 11(1): 1399

MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Chang KC, Diermeier SD, Yu AT, Brine LD, Russo S, Bhatia S, Alsudani H, Kostroff K, Bhuiya T, Brogi E, Pappin DJ, Bennett CF, Rigo F, Spector DL.

In:

Nat Commun. (2020) :

Reduced inflammation in the tumor microenvironment delays the accumulation of myeloid-derived suppressor cells and limits tumor progression

Categories:

Transfection

Authors:

Bunt SK, Yang L, Sinha P, Clements VK, Leips J, Ostrand-Rosenberg S

In:

Cancer Res (2007) 67(20): 10019-26

4T1

Transfection Information

Citations