cells

mammary carcinoma

Cell Type:

Mammary Epith.

Tissue Origin:

breast

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	T-016	1e6	60%	59%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-024	1e6	86%	51%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-027	1e6	64%	60%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	G-016	1e6	31%	78%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms

Categories:

Transfection

Authors:

Ye Wang , Morvarid Mohseni , Angelo Grauel , Javier Estrada Diez , Wei Guan , Simon Liang , Jiyoung Elizabeth Choi , Minying Pu , Dongshu Chen , Tyler Laszewski , Stephanie Schwartz , Jane Gu , Leandra Mansur , Tyler Burks , Lauren Brodeur , Roberto Velazquez , Steve Kovats , Bhavesh Pant , Giri Buruzula , Emily Deng , Julie T Chen , Farid Sari-Sarraf , Christina Dornelas , Malini Varadarajan 1, Haiyan Yu , Chen Liu , Joanne Lim , Huai-Xiang Hao , Xiaomo Jiang , Anthony Malamas , Matthew J LaMarche , Felipe Correa Geyer , Margaret McLaughlin , Carlotta Costa , Joel Wagner , David Ruddy , Pushpa Jayaraman , Nathaniel Kirkpatrick , Pu Zhang , Oleg Iartchouk , Kimberly Aardalen , Viviana Cremasco , Glenn Dranoff , Jeffrey A Engelman , Serena Silver , Hongyun Wang, William D Hastings , Silvia Goldoni

In:

Scientific Reports (2021) 11(1): 1399

Reduced inflammation in the tumor microenvironment delays the accumulation of myeloid-derived suppressor cells and limits tumor progression