4T1

mammary carcinoma

Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-016 1e6 60% 59% Plasmid (general) 2 µg 100 µl I/II/2b
V T-024 1e6 86% 51% Plasmid (general) 2 µg 100 µl I/II/2b
V T-027 1e6 64% 60% Plasmid (general) 2 µg 100 µl I/II/2b
V G-016 1e6 31% 78% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Bunt SK, Yang L, Sinha P, Clements VK, Leips J, Ostrand-Rosenberg S 
In:
Cancer Res (2007) 67(20): 10019-26