Pam212
Export
epidermal keratinocyte cell line
Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
mouse
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
T-001
2e6
30%
60%
Plasmid (general)
2 µg
100 µl
I/II/2b
T
X-001
2e6
40%
70%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact
Categories:
Transfection
Authors:
Tanoue T and Takeichi M
In:
J Cell Biol (2004) 165(4): 517-528