Neuro-2a [N2a]

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Neuroblastoma established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse.

Cell Type:
Neuroblast
Tissue Origin:
brain
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-024 1e6 66-86% 73-85% Plasmid (general) 2 µg 100 µl I/II/2b
SF 96-DS-137 2e5 65-70% 80-85% Plasmid (general) 0.4 µg 20 µl 96-well
SF DS-137 2e5 64% 70-90% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DS-137 1e6 64% 97% Plasmid (general) 2 µg 100 µl 4D X-Unit

Citations

CRISPRs for Optimal Targeting: Delivery of CRISPR Components as DNA, RNA, and Protein into Cultured Cells and Single-Cell Embryos.
Categories:
Transfection 
Authors:
Kouranova E, Forbes K, Zhao G, Warren J, Bartels A, Wu Y, Cui . 
In:
Hum Gene Ther (2016) 27(69: 464-75 
Identification of phosphotyrosine binding domain-containing proteins as novel downstream targets of the EphA8 signaling function
Categories:
Transfection 
Authors:
Shin J, Gu C, Park E, Park S 
In:
Mol Cell Biol (2007) 27(23): 8113-26 
Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy
Categories:
Transfection 
Authors:
Evgrafov OV, Mersiyanova I, Irobi J, Van Den Bosch L, Dierick I, Leung CL, Schagina O, Verpoorten N, Van Impe K, Fedotov V, Dadali E, Auer-Grumbach M, Windpassinger C, Wagner K, Mitrovic Z, Hilton-Jones D, Talbot K, Martin JJ, Vasserman N, Tverskaya S, Polyakov A, Liem RK, Gettemans J, Robberecht W, De Jonghe P and Timmerman V 
In:
Nat Genet (2004) 36(6): 602-606 
Hot-spot residue in small heat-shock protein 22 causes distal motor neuropathy
Categories:
Transfection 
Authors:
Irobi J, Van Impe K, Seeman P, Jordanova A, Dierick I, Verpoorten N, Michalik A, De Vriendt E, Jacobs A, Van Gerwen V, Vennekens K, Mazanec R, Tournev I, Hilton-Jones D, Talbot K, Kremensky I, Van Den Bosch L, Robberecht W, Van Vandekerckhove J, Broeckhoven C, Gettemans J, De Jonghe P and Timmerman V 
In:
Nat Genet (2004) 36(6): 597-601 

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