Keratinocyte, human

primary human keratinocyte

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

Our first media specialised for growing keratinocytes.
Now superceded by KGM-Gold

The original medium for growth of keratinocytes but without Calcium.

Supports proliferation of primary human keratinocytes in culture.
Drives faster proliferation than the original KGM.
Now superceded by KGM-Gold

A serum-free, calcium free keratinocyte growth medium

Optimized for Normal Human Epidermal Keratinocytes in a serum-free environment.
Provides a nutrient rich medium that provides normal physiological growth characteristics and population doubling times.
KGM-Gold supports low, moderate, and high density keratinocyte cultures better than KGM or KGM-2, without nutrient depletion.

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (11)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
von Lanzenauer SH, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R 
In:
Genes Immun (2015) 16(1): ePub 
Categories:
Transfection 
Authors:
Mulder KW, Wang X, Escriu C, Ito Y, Schwarz RF, Gillis J, Sirokmány G, Donati G, Uribe-Lewis S, Pavlidis P, Murrell A, Markowetz F, Watt FM. 
In:
Nat Cell Biol (2012) 14(7): 753-63 
Categories:
Transfection 
Authors:
Allen D, Winters E, Kenna PF, Humphries P, Jane Farrar G 
In:
J Dermatol Sci (2008) 49(3): 217-25 
Categories:
Transfection 
Authors:
Borrelli S, Testoni B, Callari M, Alotto D, Castagnoli C, Romano RA, Sinha S, Vigano AM, Mantovani R 
In:
BMC Mol Biol (2007) 8(1): 85 
Categories:
Transfection 
Authors:
Zhang JY, Adams AE, Ridky TW, Tao S, Khavari PA 
In:
Cancer Res (2007) 67(8): 3827-34 
Categories:
Transfection 
Authors:
Truong AB, Kretz M, Ridky TW, Kimmel R, Khavari PA 
In:
Genes Dev (2006) 20(22): 3185-97 
Categories:
Transfection 
Authors:
Xu Y, Shao Y, Voorhees JJ, Fisher GJ 
In:
J Biol Chem (2006) 281(37): 27389-97 
Categories:
Transfection 
Authors:
Xu Y, Tan LJ, Grachtchouk V, Voorhees JJ and Fisher GJ 
In:
J Biol Chem (2005) 280(52): 42694-42700 
Categories:
Transfection 
Authors:
Harada K, Truong AB, Cai T and Khavari PA 
In:
Mol Cell Biol (2005) 25(24): 11122-11130 
Categories:
Transfection 
Authors:
Doi H, Shibata MA, Kiyokane K and Otsuki Y 
In:
J Dermatol Sci (2003) 33: 7-16 
+ Show All