Embryonic fibroblast, chicken

primary connective tissue cell

Cell Type:
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:

Recommended Media

Medium 199 was developed in 1950 and was formulated for nutritional studies on chick embryo muscle fibroblasts. Its unique components include purines (adenine, guanine, xanthine, hypoxanthine), pyrimdines (thymine, uracil) and fat soluble compounds. Its current uses include virology and vaccine production.
These formulations require a CO2 atmosphere.

Storage = 2ºC to 8ºC

12-109 - with Hank's BSS, L-glutamine, and 1.4 g/L sodium bicarbonate
12-118 - with Hank's BSS, L-glutamine, HEPES, and 1.4 g/L sodium bicarbonate
12-117 - with Earle's BSS, L-glutamine, HEPES, and 2.2 g/L sodium bicarbonate
12-119 - with Earle's BSS, L-glutamine, and 2.2 g/L sodium bicarbonate

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic T-023 1e6 76% 50% Plasmid (general) 4 µg 100 µl I/II/2b