SMC, rat
Export
Primary rat smooth muscle cells
Cell Type:
Smooth muscle cell
Tissue Origin:
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
Smooth muscle, basic
A-033
2e6
80%
90-95%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Notch3 and IL-1beta exert opposing effects on a vascular smooth muscle cell inflammatory pathway in which NF-kappaB drives crosstalk
Categories:
Transfection
Authors:
Clement N, Gueguen M, Glorian M, Blaise R, Andreani M, Brou C, Bausero P, Limon I
In:
J Cell Sci (2007) 120(Pt 19): 3352-61
N-Terminal Proteolysis of the Endothelin B Receptor Abolishes Its Ability to Induce EGF Receptor Transactivation and Contractile Protein Expression in Vascular Smooth Muscle Cells
Categories:
Transfection
Authors:
Grantcharova E, Reusch HP, Grossmann S, Eichhorst J, Krell HW, Beyermann M, Rosenthal W, Oksche A
In:
Arterioscler Thromb Vasc Biol (2006) 26(6): 1288-96