Granule cell (CGC), mouse
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Most common neuron type in the cerebellar cortex of the vertebrate brain
Cell Type:
Neurons (Brain)
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SCN Nucleofector Solution
SCN Basic Neuro Program 4
1e5
30-40%
Plasmid (general)
0.2 µg
100 µl
I/II/2b
Citations (9)
The neurodegenerative disease protein ataxin-1 antagonizes the neuronal survival function of myocyte enhancer factor-2
Categories:
Transfection
Authors:
Bolger TA, Zhao X, Cohen TJ, Tsai CC, Yao TP
In:
J Biol Chem (2007) 282(40): 29186-92
Role of the Rho GTPase-activating protein RICS in neurite outgrowth
Categories:
Transfection
Authors:
Nasu-Nishimura Y, Hayashi T, Ohishi T, Okabe T, Ohwada S, Hasegawa Y, Senda T, Toyoshima C, Nakamura T, Akiyama T
In:
Genes Cells (2006) 11(6): 607-14
Carboxyl termini of P/Q-type Ca2+ channel 1A subunits translocate to nuclei and promote polyglutmine-mediated toxicity
Categories:
Transfection
Authors:
Kordasiewicz HB, Thompson RM, Clark HB, Gomez CM
In:
Hum Mol Genet (2006) 15(10): 1587-99
Intracellular trafficking of histone deacetylase 4 regulates neuronal cell death
Categories:
Transfection
Authors:
Bolger TA and Yao TP
In:
J Neurosci (2005) 25(41): 9544-9553
Neuronal depolarization controls brain-derived neurotrophic factor-induced upregulation of NR2C NMDA receptor via calcineurin signaling
Categories:
Transfection
Authors:
Suzuki K, Sato M, Morishima Y and Nakanishi S
In:
J Neurosci (2005) 25(41): 9535-9543
Synapse associated protein 102 is a novel binding partner to the cytoplasmic terminus of neurone-glial related cell adhesion molecule
Categories:
Transfection
Authors:
Davey F, Hill M, Falk J, Sans N and Gunn-Moore FJ
In:
J Neurochem (2005) 94(5): 1243-1253
L1-mediated branching is regulated by two ezrin-radixin-moesin (ERM)-binding sites, the RSLE region and a novel juxtamembrane ERM-binding region
Categories:
Transfection
Authors:
Cheng L, Itoh K and Lemmon V
In:
J Neurosci (2005) 25(2): 395-403
Pathological missense mutations of neural cell adhesion molecule L1 affect neurite outgrowth and branching on an L1 substrate
Categories:
Transfection
Authors:
Cheng L and Lemmon V
In:
Mol Cell Neurosci (2004) 27(4): 522-530
Par6alpha signaling controls glial-guided neuronal migration
Categories:
Transfection
Authors:
Solecki DJ, Model L, Gaetz J, Kapoor TM and Hatten ME
In:
Nat Neurosci (2004) 7(11): 1195-1203
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