RFL-6

fibroblast, Sprague-Dawley, lung

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
rat
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R G-016 1e6 30% 50% Plasmid (general) 2 µg 100 µl I/II/2b
R O-017 1e6 50-60% 75% Plasmid (general) 2 µg 100 µl I/II/2b
R T-020 1e6 50% 50% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Yu W, Qiang L, Solowska JM, Karabay A, Korulu S, Baas PW 
In:
Mol Biol Cell (2008) 19(4): 1485-98 
Categories:
Transfection 
Authors:
Qiang L, Yu W, Andreadis A, Luo M, Baas PW 
In:
J Neurosci (2006) 26(12): 3120-9 
Categories:
Transfection 
Authors:
Yu W, Solowska JM, Qiang L, Karabay A, Baird D and Baas PW 
In:
J Neurosci (2005) 25(23): 5573-5583