RFL-6
Export
fibroblast, Sprague-Dawley, lung
Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
rat
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
G-016
1e6
30%
50%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
O-017
1e6
50-60%
75%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
T-020
1e6
50%
50%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
The Microtubule-severing Proteins Spastin and Katanin Participate Differently in the Formation of Axonal Branches
Categories:
Transfection
Authors:
Yu W, Qiang L, Solowska JM, Karabay A, Korulu S, Baas PW
In:
Mol Biol Cell (2008) 19(4): 1485-98
Tau protects microtubules in the axon from severing by katanin
Categories:
Transfection
Authors:
Qiang L, Yu W, Andreadis A, Luo M, Baas PW
In:
J Neurosci (2006) 26(12): 3120-9
Regulation of microtubule severing by katanin subunits during neuronal development
Categories:
Transfection
Authors:
Yu W, Solowska JM, Qiang L, Karabay A, Baird D and Baas PW
In:
J Neurosci (2005) 25(23): 5573-5583