UACC903

melanoma

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R D-009 1e6 70-80% 55% Plasmid (general) µg 100 µl I/II/2b
R K-017 1e6 70-80% 60% Plasmid (general) µg 100 µl I/II/2b
R U-001 1e6 70-80% 75% Plasmid (general) µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Sharma A, Madhunapantula SV, Gowda R, Berg A, Neves RI, Robertson GP. 
In:
Am J Pathol (2013) 182(4): 1151-62 
Categories:
Transfection 
Authors:
Cheung M, Sharma A, Madhunapantula SV, Robertson GP 
In:
Cancer Res (2008) 68(9): 3429-39 
Categories:
Transfection 
Authors:
Liang S, Sharma A, Peng HH, Robertson G, Dong C 
In:
Cancer Res (2007) 67(12): 5814-20 
Categories:
Transfection 
Authors:
Sharma A, Tran MA, Liang S, Sharma AK, Amin S, Smith CD, Dong C, Robertson GP 
In:
Cancer Res (2006) 66(16): 8200-9 
Categories:
Transfection 
Authors:
Sharma A, Trivedi NR, Zimmerman MA, Tuveson DA, Smith CD and Robertson GP 
In:
Cancer Res (2005) 65(6): 412-421 
Categories:
Transfection 
Authors:
Stahl JM, Sharma A, Cheung M, Zimmerman M, Cheng JQ, Bosenberg MW, Kester M, Sandirasegarane L and Robertson GP 
In:
Cancer Res (2004) 64(19): 7002-7010