NCI-H295R [H295R]
Export
adrenal gland, cortex, carcinoma
Cell Type:
Adrenal Epith.
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
T-020
5e6
20%
90%
Plasmid (general)
3 µg
100 µl
I/II/2b
R
T-016
2e6
99%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
T-020
2e6
98%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Orexins stimulate steroidogenic acute regulatory (StAR) protein expression through multiple signalling pathways in human adrenal H295R cells
Categories:
Transfection
Authors:
Ramanjaneya M, Conner AC, Chen J, Stanfield PR, Randeva HS
In:
Endocrinology (2008) 149(8): 4106-15
Hint2 is expressed in the mitochondria of H295R cells and is involved in steroidogenesis
Categories:
Transfection
Authors:
Lenglet S, Antigny F, Vetterli L, Dufour JF, Rossier MF
In:
Endocrinology (2008) epub: online
MicroRNA-21 Increases Aldosterone Secretion and Proliferation in H295R Human Adrenocortical Cells
Categories:
Transfection
Authors:
Romero DG, Plonczynski MW, Carvajal CA, Gomez-Sanchez EP, Gomez-Sanchez CE
In:
Endocrinology (2008) 149(5): 2477-83
Disabled-2 is expressed in adrenal zona glomerulosa and is involved in aldosterone secretion
Categories:
Transfection
Authors:
Romero DG, Yanes LL, de Rodriguez AF, Plonczynski MW, Welsh BL, Reckelhoff JF, Gomez-Sanchez EP, Gomez-Sanchez CE
In:
Endocrinology (2007) 148(6): 2644-52
Angiotensin II-Mediated Protein Kinase D Activation Stimulates Aldosterone and Cortisol Secretion in H295R Human Adrenocortical Cells
Categories:
Transfection
Authors:
Romero DG, Welsh BL, Gomez-Sanchez EP, Yanes LL, Rilli S, Gomez-Sanchez CE
In:
Endocrinology (2006) 147(12): 6046-6055
A novel mutation in the steroidogenic acute regulatory protein gene promoter leading to reduced promoter activity
Categories:
Transfection
Authors:
Casal AJ, Sinclair VJ, Capponi AM, Nicod J, Huynh-Do U, Ferrari P
In:
J Mol Endocrinol (2006) 37(1): 71-80