NCI-H295R [H295R]

adrenal gland, cortex, carcinoma

Cell Type:
Adrenal Epith.
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Recommended Media

DMEM:F12 combines the richness of F12 with the higher component concentrations of DMEM. This medium is well suited for clonal density cultures and has been extensively used to demonstrate the effect of various hormones and growth factors on target tissues.

Storage = 2ºC to 8ºC

12-719 = 3.151 g/L glucose, L-glutamine and 15mM HEPES
BE04-687Q = 3.151 g/L glucose and L-glutamine, without HEPES
BE04-687F/U1 = 3.151 g/L glucose and Ultraglutamine I, without HEPES

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-020 5e6 20% 90% Plasmid (general) 3 µg 100 µl I/II/2b
R T-016 2e6 99% Plasmid (general) 2 µg 100 µl I/II/2b
R T-020 2e6 98% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Ramanjaneya M, Conner AC, Chen J, Stanfield PR, Randeva HS 
In:
Endocrinology (2008) 149(8): 4106-15 
Categories:
Transfection 
Authors:
Lenglet S, Antigny F, Vetterli L, Dufour JF, Rossier MF 
In:
Endocrinology (2008) epub: online 
Categories:
Transfection 
Authors:
Romero DG, Plonczynski MW, Carvajal CA, Gomez-Sanchez EP, Gomez-Sanchez CE 
In:
Endocrinology (2008) 149(5): 2477-83 
Categories:
Transfection 
Authors:
Romero DG, Yanes LL, de Rodriguez AF, Plonczynski MW, Welsh BL, Reckelhoff JF, Gomez-Sanchez EP, Gomez-Sanchez CE 
In:
Endocrinology (2007) 148(6): 2644-52 
Categories:
Transfection 
Authors:
Romero DG, Welsh BL, Gomez-Sanchez EP, Yanes LL, Rilli S, Gomez-Sanchez CE 
In:
Endocrinology (2006) 147(12): 6046-6055 
Categories:
Transfection 
Authors:
Casal AJ, Sinclair VJ, Capponi AM, Nicod J, Huynh-Do U, Ferrari P 
In:
J Mol Endocrinol (2006) 37(1): 71-80