cells

colorectal adenocarcinoma; Caco-2 was developed by the Memorial Sloan Kettering Cancer Center and is accordingly subject to certain third party rights and commercial use restrictions. Please contact MSKCC or Navicyte Scientific for licensing information.

Cell Type:

GI Tract Epith.

Tissue Origin:

colon

Species:

human

Research Area:

Cancer Research/Cell Biology

Gastroenterology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	DG-113	1e6	82% ±2	70% ±2	Plasmid (general)	2 µg	100 µl	4D X-Unit
SE	DG-113	2e5	81% ±5	80% ±4	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
T	B-024	5e5	59% ±7	70%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (14)

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

David E. Gordon*, Joseph Hiatt*, Mehdi Bouhaddou*, Veronica V. Rezelj*, Svenja Ulferts*, Hannes Braberg*,Alexander S. Jureka*, Kirsten Obernier*, Jeffrey Z.Guo*, Jyoti Batra*, Robyn M. Kaake*, Andrew R.Weckstein*,Tristan W. Owens*, Meghna Gupta*, Sergei Pourmal*, Erron W. Titus*, Merve Cakir* et al.

In:

Science (2020) 370(6521): eabe9403

Role of p38 MAP kinase and TGF-beta signaling in transepithelial migration of invasive bacterial pathogens

Categories:

Transfection

Authors:

Beisswenger C, Coyne CB, Shchepetov M, Weiser JN

In:

J Biol Chem (2007) 282(39): 28700-8

BUB1 mediation of caspase-independent mitotic death determines cell fate

Categories:

Transfection

Authors:

Niikura Y, Dixit A, Scott R, Perkins G, Kitagawa K

In:

J Cell Biol (2007) 178(2): 283-96

Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL

Categories:

Transfection

Authors:

He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, Shan M, Chadburn A, Villanacci V, Plebani A, Knowles DM, Rescigno M, Cerutti A

In:

Immunity (2007) 26(6): 812-26

Rotavirus NSP1 Inhibits Expression of Type I Interferon by Antagonizing the Function of Interferon Regulatory Factors IRF3, IRF5, and IRF7

Categories:

Transfection

Authors:

Barro M, Patton JT

In:

J Virol (2007) 81(9): 4473-81

Hsp70 negatively controls rotavirus protein bioavailability in caco-2 cells infected by the rotavirus RF strain

Categories:

Transfection

Authors:

Broquet AH, Lenoir C, Gardet A, Sapin C, Chwetzoff S, Jouniaux AM, Lopez S, Trugnan G, Bachelet M, Thomas G

In:

J Virol (2007) 81(3): 1297-304

Modulation of Human Niemann-Pick C1 like 1 Gene Expression by Sterol: Role of Sterol Regulatory Element Binding Protein-2

Categories:

Transfection

Authors:

Alrefai W, Annaba F, Sarwar Z, Dwivedi A, Saksena S, Singla A, Dudeja PK, Gill R

In:

Am J Physiol Gastrointest Liver Physiol (2007) 292(1): G369-76

Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption

Categories:

Transfection

Authors:

Qiu A, Jansen M, Sakaris A, Min SH, Chattopadhyay S, Tsai E, Sandoval C, Zhao R, Akabas MH, Goldman ID

In:

Cell (2006) 127(5): 917-28

Direct involvement of the small GTPase Rac in activation of the superoxide-producing NADPH oxidase Nox1

Categories:

Transfection

Authors:

Miyano K, Ueno N, Takeya R, Sumimoto H

In:

J Biol Chem (2006) 281(31): 21857-68

Rotavirus spike protein VP4 binds to and remodels actin bundles of the epithelial brush border into actin bodies

Categories:

Transfection

Authors:

Gardet A, Breton M, Fontanges P, Trugnan G, Chwetzoff S

In:

J Virol (2006) 80(8): 3947-56

Virus-induced Abl and Fyn kinase signals permit coxsackievirus entry through epithelial tight junctions

Categories:

Transfection

Authors:

Coyne CB, Bergelson JM

In:

Cell (2006) 124(1): 119-31

PATJ connects and stabilizes apical and lateral components of tight junctions in human intestinal cells

Categories:

Transfection

Authors:

Michel D, Arsanto JP, Massey-Harroche D, Beclin C, Wijnholds J and Le Bivic A

In:

J Cell Sci (2005) 118(Pt 17): 4049-4057

In vitro and ex vivo activation of the TLR5 signaling pathway in intestinal epithelial cells by a commensal Escherichia coli strain

Categories:

Transfection

Authors:

Bambou JC, Giraud A, Menard S, Begue B, Rakotobe S, Heyman M, Taddei F, Cerf-Bensussan N and Gaboriau-Routhiau V

In:

J Biol Chem (2004) 279(41): 42984-42992

Sequential activation of phosphatidylinositol 3-kinase, beta Pix, Rac1, and Nox1 in growth factor-induced production of H2O2

Categories:

Transfection

Authors:

Park HS, Lee SH, Park D, Lee JS, Ryu SH, Lee WJ, Rhee SG and Bae YS

In:

Mol Cell Biol (2004) 24(10): 4384-4394

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