cells

bronchial epithelial

Cell Type:

Respiratory Tract Epith.

Tissue Origin:

lung

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

Bronchial Epithelial Cell Growth Medium

Airway cell media are serum-free media that have been optimized for the proliferation of airway cells.

BEGM is optimized to provide the best growth for Normal Human Bronchial/Tracheal Epithelial Cells.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	T	G-016	1e6	30%		Plasmid (general)	2 µg	100 µl	I/II/2b
	T	G-016	1e6	80%		Plasmid (general)	2 µg	100 µl	I/II/2b

Citations (12)

Glucocorticoid Insensitivity in Virally Infected Airway Epithelial Cells Is Dependent on Transforming Growth Factor-ß Activity.

Categories:

Primary Cells and Media

Authors:

Xia YC, Radwan A, Keenan CR, Langenbach SY, Li M, Radojicic D, Londrigan SL, Gualano RC, Stewart AG

In:

PLoS Pathog (2017) 13(1): doi: 10.1371/journal.ppat.1006138

An ShRNA Based Genetic Screen Identified Sesn2 as a Potential Tumor Suppressor in Lung Cancer via Suppression of Akt-mTOR-p70S6K Signaling.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Xu H, Sun H, Zhang H, Liu J, Fan F, Li Y, Ning X, Sun Y, Dai S, Liu B, Gao M, Fu S, Zhou C

In:

PLoS ONE (2015) 10(5): e0124033

TWEAK enhances TGF-ß-induced epithelial-mesenchymal transition in human bronchial epithelial cells

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Itoigawa Y, Harada N, Harada S, Katsura Y, Makino F, Ito J, Nurwidya F, Kato M, Takahashi F, Atsuta R, Takahashi K

In:

Respir Res (2015) 16: 48

Analysis of cathepsin and furin proteolytic enzymes involved in viral fusion protein activation in cells of the bat reservoir host

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

El Najjar F, Lampe L, Baker ML, Wang LF, Dutch RE

In:

PLoS ONE (2015) 10(2): e0115736

Lung epithelial cells induce both phenotype alteration and senescence in breast cancer cells

Categories:

Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Furukawa M, Wheeler S, Clark AM, Wells A

In:

PLoS ONE (2015) 10 (1): e0118060

Particle pollution in Rio de Janeiro, Brazil: Increase and decrease of pro-inflammatory cytokines IL-6 and IL-8 in human lung cells

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Rosa I. Rodríguez-Cotto, Mario G. Ortiz-Martínez, Evasomary Rivera-Ramírez, Vinicius L. Mateus d, Beatriz S. Amaral d, Braulio D. Jimenez-Velez, Adriana Gioda

In:

Environ Pollut (2014) 194: 112-20

Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Shoaib Majeed, Stefan Frentzel, Sandra Wagner, Diana Kuehn, Patrice Leroy, Philippe A Guy, Arno Knorr, Julia Hoeng and Manuel C Peitsch

In:

Chemistry Central (2014) 8(1): 62

Rhinovirus-induced MMP-9 expression is dependent on Fra-1, which is modulated by formoterol and dexamethasone.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Tacon CE, Newton R, Proud D, Leigh R.

In:

J Immunol (2012) 88(9): 4621-30

Spleen tyrosine kinase mediates BEAS-2B cell migration and proliferation and human rhinovirus-induced expression of vascular endothelial growth factor and interleukin-8.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Xiaomin Wang, Mirek Mychajlowycz, Christine Lau, Carlos Gutierrez, Jeremy A. Scott, and Chung-Wai Chow

In:

J Pharmacol Exp Ther (2012) 240(2): 277-85

Cigarette smoke modulates rhinovirusinduced airway epithelial cell chemokine production

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

M.H. Hudy, S.L. Traves, S. Wiehler and D. Proud

In:

Eur Respir J (2010) 35(6): 1256-63

Syk associates with clathrin and mediates phosphatidylinositol 3-kinase activation during human rhinovirus internalization

Categories:

Transfection

Authors:

Lau C, Wang X, Song L, North M, Wiehler S, Proud D, Chow CW

In:

J Immunol (2008) 180(2): 870-80

Involvement of chloride channels in TGF-beta1-induced apoptosis of human bronchial epithelial cells

Categories:

Transfection

Authors:

Cheng G, Shao Z, Chaudhari BR, Agrawal DK

In:

Am J Physiol Lung Cell Mol Physiol (2007) 293(5): L1339-47

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