U373MG

astrocytoma

Cell Type:
Glial Cells
Tissue Origin:
Species:
human
Research Area:
Neurobiology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-020 1e6 70% 99% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ikushima H, Todo T, Ino Y, Takahashi M, Saito N, Miyazawa K, Miyazono K. 
In:
J Biol Chem (2011) 286(48): 41434-41441 
Categories:
Transfection 
Authors:
Gopalan SM, Wilczynska KM, Konik BS, Bryan L and Kordula T 
In:
J Biol Chem (2006) 281(4): 1956-1963