cells

myeloma

Cell Type:

B cell

Tissue Origin:

Species:

mouse

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CQ-104	1e6	68-70%	74-86%	Plasmid (general)	2 µg	100 µl	4D X-Unit
SF	CQ-104	2e5	63-67%	84-96%	Plasmid (general)	400 ng	20 µl	4D X-Unit
R	X-001	2e6	71%	80-90%	Plasmid (general)	µg	100 µl	I/II/2b
R	X-005	2e6	64%	80-90%	Plasmid (general)	µg	100 µl	I/II/2b
R	B-033	2e6	52%	80-90%	Plasmid (general)	µg	100 µl	I/II/2b
R	A-033	2e6	37%	80-90%	Plasmid (general)	µg	100 µl	I/II/2b
R	T-020	2e6	52%	80-90%	Plasmid (general)	µg	100 µl	I/II/2b

SP2/0

Transfection Information