Mouse L cell

Fibroblast line

Cell Type:
Tissue Origin:
Connective tissue proper
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:

Recommended Media

Basal Medium Eagle is a minimal medium suitable for a variety of cell types.  It is the historical precursor to Minimal Essential Medium (MEM) and was was formulated in 1955 for nutritional requirements of HeLa and mouse L cells.  BME can be used for culturing primary or diploid mammalian cells.

12-105 = BME with Earle's BSS, without L-glutamine 

Storage = 15ºC to 30ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-020 2e6 20-50% 95% Plasmid (general) µg 100 µl I/II/2b
T U-024 2e6 20-50% 95% Plasmid (general) µg 100 µl I/II/2b