OVCAR3

ovarian carcinoma; epithelial, adenocarcinoma

Cell Type:
Ovary Epith.
Tissue Origin:
ovarian
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-016 8e5 80% 90% Plasmid (general) 2 µg 100 µl I/II/2b
T T-027 8e5 60% 70% Plasmid (general) 2 µg 100 µl I/II/2b
T A-023 8e5 60% 70% Plasmid (general) 2 µg 100 µl I/II/2b
R A-023 2e6 60% 95% Plasmid (general) 4 µg 100 µl I/II/2b
R T-016 2e6 70% 95% Plasmid (general) 4 µg 100 µl I/II/2b
R O-017 2e6 70% 92% Plasmid (general) 4 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Sheu JJ, Choi JH, Yildiz I, Tsai FJ, Shaul Y, Wang TL, Shih IeM 
In:
Cancer Res (2008) 68(11): 4050-7 
Categories:
Transfection 
Authors:
Morrison BH, Bauer JA, Lupica JA, Tang Z, Schmidt H, Didonato JA, Lindner DJ 
In:
J Biol Chem (2007) 282(21): 15349-56 
Categories:
Transfection 
Authors:
Tang Z, Bauer JA, Morrison B, Lindner DJ 
In:
Mol Cell Biol (2006) 26(15): 5588-94 
Categories:
Transfection 
Authors:
Chen YC, Pohl G, Wang TL, Morin PJ, Risberg B, Kristensen GB, Yu A, Davidson B and Shih IeM 
In:
Cancer Res (2005) 65(1): 331-337 
Categories:
Transfection 
Authors:
Cheng KW, Lahad JP, Kuo WL, Lapuk A, Yamada K, Auersperg N, Liu J, Smith-McCune K, Lu KH, Fishman D, Gray JW and Mills GB 
In:
Nat Med (2004) 10(11): 1251-1256