Hepatocyte immortalized, mouse
Export
SV 40 immortalized murine hepatocytes
Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
T-020
1e6
70%
45%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-016
1e6
37%
55%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Developmental Switch from Prolonged Insulin Action to Increased Insulin Sensitivity in Protein Tyrosine Phosphatase 1B-Deficient Hepatocytes
Categories:
Transfection
Authors:
Gonzalez-Rodriguez A, Clampit JE, Escribano O, Benito M, Rondinone CM, Valverde AM
In:
Endocrinology (2007) 148(2): 594-608