Endothelial, MV dermal (HMVEC-d), human

Primary endothelium from human dermal microvasculature

Cell Type:
Microvascular Endo.
Tissue Origin:
dermal
Species:
human
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Endothelial, basic S-005 6e5 80% poor Plasmid (general) 2 µg 100 µl I/II/2b
Endothelial, basic M-003 6e5 40% moderate Plasmid (general) 2 µg 100 µl I/II/2b
HMVEC-L S-005 5e5 70% 90% Plasmid (general) 3 µg 100 µl I/II/2b

Citations (25)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ruiz MA, Feng B, Chakrabarti S 
In:
PLoS ONE (2015) 10(4): e0123987 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lopatina T, Bruno S, Tetta C, Kalinina N, Porta M, Camussi G 
In:
Cell Commun Signal (2014) 12: ePub 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Speyer CL1, Hachem AH2, Assi AA2, Johnson JS3, DeVries JA4, Gorski DH5. 
In:
PLoS ONE (2014) 9(3):: e88830. 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Klein JD, Sano D, Sen M, Myers JN, Grandis JR, Kim S 
In:
PLoS ONE (2014) 9(1): e81819 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Patel H1, Chen J, Das KC, Kavdia M. 
In:
Cardiovasc Diabetol (2013) 12: 142 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vervaeke P1, Alen M, Noppen S, Schols D, Oreste P, Liekens S. 
In:
PLoS ONE (2013) 8(8): e74035 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Todorovich SM, Khan ZA 
In:
Other (2013) 5(3): 352-7 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Peshavariya HM, Taylor CJ, Goh C, Liu GS, Jiang F, Chan EC, Dusting GJ. 
In:
PLoS ONE (2013) 8(4): e60790 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Daniel A. Dworkis1,2, Elizabeth S. Klings1, Sherene M. Shenouda, Nadia Solovieff, Efthymia Melista, Carl Giovannucci, Surinder Safaya, Guihua Li, Joseph A. Vita, Martin H. Steinberg, Clinton T. Baldwin 
In:
Open Journal of Blood Diseases (2013) 3: 25-31 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mortuza R, Chen S, Feng B, Sen S, Chakrabarti S 
In:
PLoS ONE (2013) 8(1): e54514 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Liu JJ, Stockton RA, Gingras AR, Ablooglu AJ, Han J, Bobkov AA, Ginsberg MH. 
In:
Mol Biol Cell (2011) 22(14): 2509-19 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang Y, Sathish N, Hollow C, Yuan Y. 
In:
J Virol (2011) 85(5): 1943-57 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yasuniwa Y, Izumi H, Wang KY, Shimajiri S, Sasaguri Y, Kawai K, Kasai H, Shimada T, Miyake K, Kashiwagi E, Hirano G, Kidani A, Akiyama M, Han B, Wu Y, Ieiri I, Higuchi S, Kohno K. 
In:
PLoS ONE (2010) 5(12): e15330 
Categories:
Primary Cells and Media 
Authors:
Shahani T, Lavend\'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. 
In:
Blood (2010) 115(23): 4902-9 
Categories:
Primary Cells and Media 
Authors:
Fitchev PP, Wcislak SM, Lee C, Bergh A, Brendler CB, Stellmach VM, Crawford SE, Mavroudis CD, Cornwell ML, Doll JA. 
In:
Lab Invest (2010) 90(7): 1078-90 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Grote K, Schuett H, Salguero G, Grothusen C, Jagielska J, Drexler H, Mühlradt PF, Schieffer B. 
In:
Blood (2010) 115(12): 2543-52 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Andreeva AV, Kutuzov MA, Tkachuk VA, Voyno-Yasenetskaya TA. 
In:
Am J Physiol Cell Physiol (2009) 297(5): C1168-77 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alfano RW1, Leppla SH, Liu S, Bugge TH, Meininger CJ, Lairmore TC, Mulne AF, Davis SH, Duesbery NS, Frankel AE. 
In:
Mol Cancer Res (2009) Apr;7(4): 452-61 
Categories:
Transfection 
Authors:
Fritz-Six KL, Dunworth WP, Li M, Caron KM 
In:
J Clin Invest (2008) 118(1): 40-50 
Categories:
Transfection 
Authors:
Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergun S 
In:
Blood (2007) 110(13): 4223-33 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mulligan-Kehoe MJ, Drinane MC, Mollmark J, Casciola-Rosen L, Hummers LK, Hall A, Rosen A, Wigley FM, Simons M. 
In:
Arthritis Rheum (2007) 56(10): 3448-58 
Categories:
Transfection 
Authors:
Meng Q, Lux A, Holloschi A, Li J, Hughes JM, McCarthy JE, Heagerty AM, Kioschis P, Hafner M, Garland JM 
In:
J Biol Chem (2006) 281(48): 37069-80 
Categories:
Transfection 
Authors:
Orlova VV, Economopoulou M, Lupu F, Santoso S, Chavakis T 
In:
J Exp Med (2006) 203(12): 2703-14 
Categories:
Transfection 
Authors:
Kilic N, Oliviera-Ferrer L, Loges S, Chalajour F, Weil J, Fernando M, Ergun S 
In:
J Biol Chem (2005) 280(3): 2361-2369 
Categories:
Transfection 
Authors:
Adini I, Rabinovitz I, Sun JF, Prendergast GC and Benjamin LE 
In:
Genes Dev (2003) 17: 2721-2732 
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