Endothelial, MV dermal (HMVEC-d), human

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Primary endothelium from human dermal microvasculature

Cell Type:
Microvascular Endo.
Tissue Origin:
dermal
Species:
human
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

Endothelial Cell Growth Medium-2 Microvascular

EGM-2 MV has been developed for the enhanced growth of lung microvascular endothelial cells
Can be used to grow all Clonetics Endothelial cells except HUVEC
Final serum concentration is 5%
Does not contain BBE

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Endothelial, basic S-005 6e5 80% poor Plasmid (general) 2 µg 100 µl I/II/2b
Endothelial, basic M-003 6e5 40% moderate Plasmid (general) 2 µg 100 µl I/II/2b
HMVEC-L S-005 5e5 70% 90% Plasmid (general) 3 µg 100 µl I/II/2b

Citations (26)

A glass-based, continuously zonated and vascularized human liver acinus microphysiological system (vLAMPS) designed for experimental modeling of diseases and ADME/TOX.
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Li X, George SM, Vernetti L, Gough AH, Taylor DL. 
In:
Lab Chip (2018) 18(17): 2614-2631 
Polycomb repressive complex 2 regulates MiR-200b in retinal endothelial cells: potential relevance in diabetic retinopathy.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ruiz MA, Feng B, Chakrabarti S 
In:
PLoS ONE (2015) 10(4): e0123987 
Platelet-derived growth factor regulates the secretion of extracellular vesicles by adipose mesenchymal stem cells and enhances their angiogenic potential
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Lopatina T, Bruno S, Tetta C, Kalinina N, Porta M, Camussi G 
In:
Cell Commun Signal (2014) 12: ePub 
Metabotropic glutamate receptor-1 as a novel target for the antiangiogenic treatment of breast cancer.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Speyer CL1, Hachem AH2, Assi AA2, Johnson JS3, DeVries JA4, Gorski DH5. 
In:
PLoS ONE (2014) 9(3):: e88830. 
STAT3 oligonucleotide inhibits tumor angiogenesis in preclinical models of squamous cell carcinoma
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Klein JD, Sano D, Sen M, Myers JN, Grandis JR, Kim S 
In:
PLoS ONE (2014) 9(1): e81819 
Hyperglycemia induces differential change in oxidative stress at gene expression and functional levels in HUVEC and HMVEC.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Patel H1, Chen J, Das KC, Kavdia M. 
In:
Cardiovasc Diabetol (2013) 12: 142 
Sulfated Escherichia coli K5 polysaccharide derivatives inhibit dengue virus infection of human microvascular endothelial cells by interacting with the viral envelope protein E domain III.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vervaeke P1, Alen M, Noppen S, Schols D, Oreste P, Liekens S. 
In:
PLoS ONE (2013) 8(8): e74035 
Elevated T-box 2 in infantile hemangioma stem cells maintains an adipogenic differentiation-competent state.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Todorovich SM, Khan ZA 
In:
Other (2013) 5(3): 352-7 
Annexin peptide Ac2-26 suppresses TNFa-induced inflammatory responses via inhibition of Rac1-dependent NADPH oxidase in human endothelial cells
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Peshavariya HM, Taylor CJ, Goh C, Liu GS, Jiang F, Chan EC, Dusting GJ. 
In:
PLoS ONE (2013) 8(4): e60790 
ARFGEF2 Knockdown Enhances TNF-a Induced Endothelial Expression of the Cell Adhesion Molecules VCAM1 and ICAM1*
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Daniel A. Dworkis1,2, Elizabeth S. Klings1, Sherene M. Shenouda, Nadia Solovieff, Efthymia Melista, Carl Giovannucci, Surinder Safaya, Guihua Li, Joseph A. Vita, Martin H. Steinberg, Clinton T. Baldwin 
In:
Open Journal of Blood Diseases (2013) 3: 25-31 
High glucose induced alteration of SIRTs in endothelial cells causes rapid aging in a p300 and FOXO regulated pathway
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mortuza R, Chen S, Feng B, Sen S, Chakrabarti S 
In:
PLoS ONE (2013) 8(1): e54514 
A mechanism of Rap1-induced stabilization of endothelial cell--cell junctions.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Liu JJ, Stockton RA, Gingras AR, Ablooglu AJ, Han J, Bobkov AA, Ginsberg MH. 
In:
Mol Biol Cell (2011) 22(14): 2509-19 
Functional Characterization of Kaposi’s Sarcoma-Associated Herpesvirus Open Reading Frame K8 by Bacterial Artificial Chromosome-Based Mutagenesis
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Wang Y, Sathish N, Hollow C, Yuan Y. 
In:
J Virol (2011) 85(5): 1943-57 
Circadian disruption accelerates tumor growth and angio/stromagenesis through a Wnt signaling pathway.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Yasuniwa Y, Izumi H, Wang KY, Shimajiri S, Sasaguri Y, Kawai K, Kasai H, Shimada T, Miyake K, Kashiwagi E, Hirano G, Kidani A, Akiyama M, Han B, Wu Y, Ieiri I, Higuchi S, Kohno K. 
In:
PLoS ONE (2010) 5(12): e15330 
Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool
Categories:
Primary Cells and Media 
Authors:
Shahani T, Lavend\'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. 
In:
Blood (2010) 115(23): 4902-9 
Thrombospondin-1 regulates the normal prostate in vivo through angiogenesis and TGF-beta activation.
Categories:
Primary Cells and Media 
Authors:
Fitchev PP, Wcislak SM, Lee C, Bergh A, Brendler CB, Stellmach VM, Crawford SE, Mavroudis CD, Cornwell ML, Doll JA. 
In:
Lab Invest (2010) 90(7): 1078-90 
Toll-like receptor 2/6 stimulation promotes angiogenesis via GM-CSF as a potential strategy for immune defense and tissue regeneration.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Grote K, Schuett H, Salguero G, Grothusen C, Jagielska J, Drexler H, Mühlradt PF, Schieffer B. 
In:
Blood (2010) 115(12): 2543-52 
T-cadherin is located in the nucleus and centrosomes in endothelial cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Andreeva AV, Kutuzov MA, Tkachuk VA, Voyno-Yasenetskaya TA. 
In:
Am J Physiol Cell Physiol (2009) 297(5): C1168-77 
Matrix metalloproteinase-activated anthrax lethal toxin inhibits endothelial invasion and neovasculature formation during in vitro morphogenesis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Alfano RW1, Leppla SH, Liu S, Bugge TH, Meininger CJ, Lairmore TC, Mulne AF, Davis SH, Duesbery NS, Frankel AE. 
In:
Mol Cancer Res (2009) Apr;7(4): 452-61 
Adrenomedullin signaling is necessary for murine lymphatic vascular development
Categories:
Transfection 
Authors:
Fritz-Six KL, Dunworth WP, Li M, Caron KM 
In:
J Clin Invest (2008) 118(1): 40-50 
Lymphatic reprogramming of micro vascular endothelial cells by CEA-related Cell Adhesion Molecule-1 via interaction with VEGFR-3 and Prox1
Categories:
Transfection 
Authors:
Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergun S 
In:
Blood (2007) 110(13): 4223-33 
Antiangiogenic plasma activity in patients with systemic sclerosis.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Mulligan-Kehoe MJ, Drinane MC, Mollmark J, Casciola-Rosen L, Hummers LK, Hall A, Rosen A, Wigley FM, Simons M. 
In:
Arthritis Rheum (2007) 56(10): 3448-58 
Identification of Tctex2beta a novel dynein light chain family member interacting with different TGF-beta receptors
Categories:
Transfection 
Authors:
Meng Q, Lux A, Holloschi A, Li J, Hughes JM, McCarthy JE, Heagerty AM, Kioschis P, Hafner M, Garland JM 
In:
J Biol Chem (2006) 281(48): 37069-80 
Junctional adhesion molecule-C regulates vascular endothelial permeability by modulating VE-cadherin-mediated cell-cell contacts
Categories:
Transfection 
Authors:
Orlova VV, Economopoulou M, Lupu F, Santoso S, Chavakis T 
In:
J Exp Med (2006) 203(12): 2703-14 
Pro-angiogenic signaling by endothelial presence of CEA-related cell adhesion molecule-1 (CEACAM1)
Categories:
Transfection 
Authors:
Kilic N, Oliviera-Ferrer L, Loges S, Chalajour F, Weil J, Fernando M, Ergun S 
In:
J Biol Chem (2005) 280(3): 2361-2369 
RhoB controls Akt trafficking and stage-specific survival of endothelial cells during vascular development
Categories:
Transfection 
Authors:
Adini I, Rabinovitz I, Sun JF, Prendergast GC and Benjamin LE 
In:
Genes Dev (2003) 17: 2721-2732 
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