H4

neuroglioma, epithelial like

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
human
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SG CM-113 2e5 79% 79-81% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SG CM-113 1e6 77-79% 79-83% Plasmid (general) 2 µg 100 µl 4D X-Unit
V T-016 1e6 75% 80% Plasmid (general) 2 µg 100 µl I/II/2b
V O-017 1e6 70% 90% Plasmid (general) 2 µg 100 µl I/II/2b
V T-020 1e6 30% 90% Plasmid (general) 2 µg 100 µl I/II/2b
V T-027 1e6 80% 80% Plasmid (general) 2 µg 100 µl I/II/2b
SG DS-138 2e5 78% Plasmid (general) 400 ng 20 µl Shuttle
SG FF-120 2e5 72% Plasmid (general) 400 ng 20 µl Shuttle

Citations

Categories:
Transfection 
Authors:
Zhang G, Dong Y, Zhang B, Ichinose F, Wu X, Culley DJ, Crosby G, Tanzi RE, Xie Z 
In:
J Neurosci (2008) 28(17): 4551-60 
Categories:
Transfection 
Authors:
Huttunen HJ, Greco C, Kovacs DM 
In:
FEBS Lett (2007) 581(8): 1688-92 
Categories:
Transfection 
Authors:
Xie Z, Dong Y, Maeda U, Xia W, Tanzi RE 
In:
J Biol Chem (2007) 282(7): 4318-4325 
Categories:
Transfection 
Authors:
Hiltunen M, Lu A, Thomas AV, Romano DM, Kim M, Jones PB, Xie Z, Kounnas MZ, Wagner SL, Berezovska O, Hyman BT, Tesco G, Bertram L, Tanzi RE 
In:
J Biol Chem (2006) 281(43): 32240-32253 
Categories:
Transfection 
Authors:
Romano DM, Dong Y, Hiltunen M, Tanzi RE, Xie Z 
In:
Amyloid (2006) 13(2): 86-92 
Categories:
Transfection 
Authors:
Xie Z, Romano DM and Tanzi RE 
In:
J Biol Chem (2005) 280(15): 15413-15421