cells

SV40-immortalized, nontransformed dermal fibroblasts from 18 year old male with Ataxia Telangiectasia (AT), a DNA repair disorder characterized by sensitivity to ionizing radiation and increased cancer risk.

Cell Type:

Fibroblast

Tissue Origin:

dermal

Species:

human

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	U-020	1e6	30-40%	80%	Plasmid (general)	5 µg	100 µl	I/II/2b
	V	P-022	1e6	30-40%	80%	Plasmid (general)	5 µg	100 µl	I/II/2b

GM05849

Transfection Information