SV40-immortalized, nontransformed dermal fibroblasts from 18 year old male with Ataxia Telangiectasia (AT), a DNA repair disorder characterized by sensitivity to ionizing radiation and increased cancer risk.

Cell Type:
Tissue Origin:
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V U-020 1e6 30-40% 80% Plasmid (general) 5 µg 100 µl I/II/2b
V P-022 1e6 30-40% 80% Plasmid (general) 5 µg 100 µl I/II/2b