Colo205
Export
human colon adenocarcinoma
Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
A-024
1e6
68%
70%
Plasmid (general)
10 µg
100 µl
I/II/2b
T
A-013
1e6
55%
80%
Plasmid (general)
10 µg
100 µl
I/II/2b
V
A-024
1e6
40%
70%
Plasmid (general)
10 µg
100 µl
I/II/2b
V
A-013
1e6
30%
80%
Plasmid (general)
10 µg
100 µl
I/II/2b
SG
DP-113
2e5
57-59%
69-75%
Plasmid (general)
1 µg
20 µl
4D X-Unit
SG
DP-113
1e6
53%
63-65%
Plasmid (general)
5 µg
100 µl
4D X-Unit
Citations
V9V2 T Cell Response to Colon Carcinoma Cells
Categories:
Transfection
Authors:
Corvaisier M, Moreau-Aubry A, Diez E, Bennouna J, Mosnier JF, Scotet E, Bonneville M and Jotereau F
In:
J Immunol (2005) 175(8): 5481-5488