HTC

rat liver hepatoma

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Recommended Media

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V V-020 1e6 75% 70% Plasmid (general) 5 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Barfod ET, Moore AL, Roe MW, Lidofsky SD 
In:
J Biol Chem (2007) 282(12): 8984-93 
Categories:
Transfection 
Authors:
Ichijo T, Voutetakis A, Cotrim AP, Bhattachryya N, Fujii M, Chrousos GP and Kino T 
In:
J Biol Chem (2005) 280(51): 42067-42077 
Categories:
Transfection 
Authors:
Kino T, Tiulpakov A, Ichijo T, Chheng L, Kozasa T and Chrousos GP 
In:
J Cell Biol (2005) 169(6): 885-896