cells

rat liver hepatoma

Cell Type:

Liver Epith.

Tissue Origin:

liver

Species:

rat

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	V-020	1e6	75%	70%	Plasmid (general)	5 µg	100 µl	I/II/2b

Citations

Ca2+-activated IK1 channels associate with lipid rafts upon cell swelling and mediate volume recovery

Categories:

Transfection

Authors:

Barfod ET, Moore AL, Roe MW, Lidofsky SD

In:

J Biol Chem (2007) 282(12): 8984-93

The Smad6-histone deacetylase 3 complex silences the transcriptional activity of the glucocorticoid receptor: potential clinical implications

Categories:

Transfection

Authors:

Ichijo T, Voutetakis A, Cotrim AP, Bhattachryya N, Fujii M, Chrousos GP and Kino T

In:

J Biol Chem (2005) 280(51): 42067-42077

G protein interacts with the glucocorticoid receptor and suppresses its transcriptional activity in the nucleus

Categories:

Transfection

Authors:

Kino T, Tiulpakov A, Ichijo T, Chheng L, Kozasa T and Chrousos GP

In:

J Cell Biol (2005) 169(6): 885-896

HTC

Transfection Information

Citations