HuH7

human hepatoma cell line

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-014 4e6 60% good Plasmid (general) 4 µg 100 µl I/II/2b
T T-022 4e6 60% good Plasmid (general) 4 µg 100 µl I/II/2b
T T-016 4e6 60% good Plasmid (general) 4 µg 100 µl I/II/2b
T T-020 2e6 60% 95% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Cell Lines and Primary Cancer Cells 
Authors:
Markus Hoffmann  1 , Hannah Kleine-Weber  2 , Simon Schroeder  3 , Nadine Krüger  4 , Tanja Herrler  5 , Sandra Erichsen  6 , Tobias S Schiergens  7 , Georg Herrler  8 , Nai-Huei Wu  8 , Andreas Nitsche  9 , Marcel A Müller  10 , Christian Drosten  3 , Stefan Pöhlmann  11              
In:
Cell (2020) 1: 1 
Categories:
Transfection 
Authors:
Hung JH, Lu YS, Wang YC, Ma YH, Wang DS, Kulp SK, Muthusamy N, Byrd JC, Cheng AL, Chen CS 
In:
Cancer Res (2008) 68(4): 1204-12 
Categories:
Transfection 
Authors:
Tani H, Komoda Y, Matsuo E, Suzuki K, Hamamoto I, Yamashita T, Moriishi K, Fujiyama K, Kanto T, Hayashi N, Owsianka A, Patel AH, Whitt MA, Matsuura Y 
In:
J Virol (2007) 81(16): 8601-12 
Categories:
Transfection 
Authors:
Cantarini MC, de la Monte SM, Pang M, Tong M, D'Errico A, Trevisani F, Wands JR 
In:
Hepatology (2006) 44(2): 446-57