HuH7

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human hepatoma cell line

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-014 4e6 60% good Plasmid (general) 4 µg 100 µl I/II/2b
T T-022 4e6 60% good Plasmid (general) 4 µg 100 µl I/II/2b
T T-016 4e6 60% good Plasmid (general) 4 µg 100 µl I/II/2b
T T-020 2e6 60% 95% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (7)

Establishment of a cell culture platform for human liver organoids and its application for lipid metabolism research
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Ayane Kuboyama-Sasaki , Yu Takahashi , Chen Xia , Kahori Hiro , Tsuyoshi Kobayashi , Hideki Ohdan , Makoto Shimizu , Yoshio Yamauchi , Hiroshi Kiyono , Ryuichiro Sato  
In:
Biotechnology Journal (2024) 19: 1 
Identify Host Factors for SARS-CoV-2 and Common Cold Coronaviruses
Categories:
Transfection 
Authors:
Wang R, Simoneau CR, Kulsuptrakul J, Bouhaddou M, Travisano KA, Hayashi JM, Carlson-Stevermer J, Zengel JR, Richards CM, Fozouni P, Oki J, Rodriguez L, Joehnk B, Walcott K, Holden K, Sil A, Carette JE, Krogan NJ, Ott M, Puschnik AS 
In:
Cell (2021) Jan 7;184(1): 106-119.e14. 
Hepatitis Delta Virus Acts as an Immunogenic Adjuvant in Hepatitis B Virus-Infected Hepatocytes
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Christine Y.L. Tham, Janine Kah, Anthony T. Tan, ..., Marc L€utgehetmann, Maura Dandri, Antonio Bertoletti 
In:
Cell Rep (2020) 1 (4): 100060 
SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor 
Categories:
Cell Lines and Primary Cancer Cells 
Authors:
Markus Hoffmann  1 , Hannah Kleine-Weber  2 , Simon Schroeder  3 , Nadine Krüger  4 , Tanja Herrler  5 , Sandra Erichsen  6 , Tobias S Schiergens  7 , Georg Herrler  8 , Nai-Huei Wu  8 , Andreas Nitsche  9 , Marcel A Müller  10 , Christian Drosten  3 , Stefan Pöhlmann  11              
In:
Cell (2020) 1: 1 
FTY720 induces apoptosis in hepatocellular carcinoma cells through activation of protein kinase C delta signaling
Categories:
Transfection 
Authors:
Hung JH, Lu YS, Wang YC, Ma YH, Wang DS, Kulp SK, Muthusamy N, Byrd JC, Cheng AL, Chen CS 
In:
Cancer Res (2008) 68(4): 1204-12 
Replication-competent recombinant vesicular stomatitis virus encoding hepatitis C virus envelope proteins
Categories:
Transfection 
Authors:
Tani H, Komoda Y, Matsuo E, Suzuki K, Hamamoto I, Yamashita T, Moriishi K, Fujiyama K, Kanto T, Hayashi N, Owsianka A, Patel AH, Whitt MA, Matsuura Y 
In:
J Virol (2007) 81(16): 8601-12 
Aspartyl-asparagyl beta hydroxylase over-expression in human hepatoma is linked to activation of insulin-like growth factor and notch signaling mechanisms
Categories:
Transfection 
Authors:
Cantarini MC, de la Monte SM, Pang M, Tong M, D'Errico A, Trevisani F, Wands JR 
In:
Hepatology (2006) 44(2): 446-57 
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