AML

Acute Myeloid Leukemia - An acute form of leukaemia, a malignant disease of the white blood cells affecting monocytes or granulocytes. It is characterised by the appearance of immature, abnormal cells in the bone marrow and peripheral blood.

Cell Type:
Myeloid (unspec.)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
T T-001 5e6 55% 86% Plasmid (general) µg 100 µl I/II/2b
V T-001 5e6 49% 87% Plasmid (general) µg 100 µl I/II/2b
V O-017 5e6 52% 66% Plasmid (general) µg 100 µl I/II/2b
T T-016 5e6 30% 52% Plasmid (general) 1 µg 100 µl I/II/2b
T T-020 5e6 56% 45% Plasmid (general) 1 µg 100 µl I/II/2b
T T-027 5e6 41% 50% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (10)

Categories:
Primary Cells and Media 
Authors:
Reikvam H, Nepstad I, Bruserud Ø, Hatfield KJ 
In:
OncoTarget (2013) 4(6): 830-43 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Rizo A, Olthof S, Han L, Vellenga E, de Haan G, Schuringa JJ 
In:
Blood (2009) 114(8): 1498-505 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schepers H, van Gosliga D, Wierenga AT, Eggen BJ, Schuringa JJ, Vellenga E 
In:
Blood (2007) 110(8): 2880-8 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Schepers H, Wierenga AT, van Gosliga D, Eggen BJ, Vellenga E, Schuringa JJ 
In:
Blood (2007) 110(4): 1317-25 
Categories:
Transfection 
Authors:
Billottet C, Grandage VL, Gale RE, Quattropani A, Rommel C, Vanhaesebroeck B, Khwaja A 
In:
Oncogene (2006) 25(50): 6648-59 
Categories:
Transfection 
Authors:
Grandage VL, Gale RE, Linch DC and Khwaja A 
In:
Leukemia (2005) 19(4): 586-594 
Categories:
Transfection 
Authors:
Simon M, Grandage VL, Linch DC and Khwaja A 
In:
Oncogene (2005) 24(14): 2410-2420 
Categories:
Serum-free and Speciality Media 
Authors:
Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F. 
In:
Mol Ther (2005) 11(1): 120-131 
Categories:
Transfection 
Authors:
Schakowski F, Buttgereit P, Mazur M, Marten A, Schottker B, Gorschluter M and Schmidt-Wolf IG 
In:
Genet Vaccines Ther (2004) 2(1): 1 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bruserud Ø, Hovland R, Wergeland L, Huang TS, Gjertsen BT. 
In:
Haematologica (2003) 88(4): 416-28 
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