cells

Acute Myeloid Leukemia - An acute form of leukaemia, a malignant disease of the white blood cells affecting monocytes or granulocytes. It is characterised by the appearance of immature, abnormal cells in the bone marrow and peripheral blood.

Cell Type:

Myeloid (unspec.)

Tissue Origin:

blood

Species:

human

Research Area:

Cancer Research/Cell Biology

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
T	T-001	5e6	55%	86%	Plasmid (general)	µg	100 µl	I/II/2b
V	T-001	5e6	49%	87%	Plasmid (general)	µg	100 µl	I/II/2b
V	O-017	5e6	52%	66%	Plasmid (general)	µg	100 µl	I/II/2b
T	T-016	5e6	30%	52%	Plasmid (general)	1 µg	100 µl	I/II/2b
T	T-020	5e6	56%	45%	Plasmid (general)	1 µg	100 µl	I/II/2b
T	T-027	5e6	41%	50%	Plasmid (general)	1 µg	100 µl	I/II/2b

Citations (9)

Repression of BMI1 in normal and leukemic human CD34(+) cells impairs self-renewal and induces apoptosis

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Rizo A, Olthof S, Han L, Vellenga E, de Haan G, Schuringa JJ

In:

Blood (2009) 114(8): 1498-505

STAT5 is required for long-term maintenance of normal and leukemic human stem/progenitor cells

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Schepers H, van Gosliga D, Wierenga AT, Eggen BJ, Schuringa JJ, Vellenga E

In:

Blood (2007) 110(8): 2880-8

Reintroduction of C/EBPalpha in leukemic CD34+ stem/progenitor cells impairs self-renewal and partially restores myelopoiesis

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Schepers H, Wierenga AT, van Gosliga D, Eggen BJ, Vellenga E, Schuringa JJ

In:

Blood (2007) 110(4): 1317-25

A selective inhibitor of the p110delta isoform of PI 3-kinase inhibits AML cell proliferation and survival and increases the cytotoxic effects of VP16

Categories:

Transfection

Authors:

Billottet C, Grandage VL, Gale RE, Quattropani A, Rommel C, Vanhaesebroeck B, Khwaja A

In:

Oncogene (2006) 25(50): 6648-59

PI3-kinase/Akt is constitutively active in primary acute myeloid leukaemia cells and regulates survival and chemoresistance via NF-kB, MAPkinase and p53 pathways

Categories:

Transfection

Authors:

Grandage VL, Gale RE, Linch DC and Khwaja A

In:

Leukemia (2005) 19(4): 586-594

Constitutive activation of the Wnt/beta-catenin signalling pathway in acute myeloid leukaemia

Categories:

Transfection

Authors:

Simon M, Grandage VL, Linch DC and Khwaja A

In:

Oncogene (2005) 24(14): 2410-2420

IL-2/B7.1 (CD80) fusagene transduction of AML blasts by a self-inactivating lentiviral vector stimulates T cell responses in vitro: a strategy to generate whole cell vaccines for AML.

Categories:

Serum-free and Speciality Media

Authors:

Chan L, Hardwick N, Darling D, Galea-Lauri J, Gäken J, Devereux S, Kemeny M, Mufti G, Farzaneh F.

In:

Mol Ther (2005) 11(1): 120-131

Novel non-viral method for transfection of primary leukemia cells and cell lines

Categories:

Transfection

Authors:

Schakowski F, Buttgereit P, Mazur M, Marten A, Schottker B, Gorschluter M and Schmidt-Wolf IG

In:

Genet Vaccines Ther (2004) 2(1): 1

Flt3-mediated signaling in human acute myelogenous leukemia (AML) blasts: a functional characterization of Flt3-ligand effects in AML cell populations with and without genetic Flt3 abnormalities.

Categories:

Classical Media and Reagents, Serum-free and Speciality Media

Authors:

Bruserud Ø, Hovland R, Wergeland L, Huang TS, Gjertsen BT.

In:

Haematologica (2003) 88(4): 416-28

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