Cardiomyocyte (R-CM), rat

Neonatal heart muscle cells

Cell Type:
Cardiac Muscle
Tissue Origin:
heart
Species:
rat
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Recommended Media

RCGM is specifically formulated for the growth and survival of rat neonatal cardiac myocytes in culture.

RCGM Medium must be further supplemented with BrdU (CC-4519 sold separately) to prevent the overgrowth of fibroblasts.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Cardiomyocyte, rat, neo G-009 2e6 31-39% 50-60% Plasmid (general) 2.5 µg 100 µl I/II/2b

Citations (28)

Categories:
Primary Cells and Media 
Authors:
Tomasovic A, Brand T, Schanbacher C, Kramer S, Hümmert MW, Godoy P, Schmidt-Heck W, Nordbeck P, Ludwig J, Homann S, Wiegering A, Shaykhutdinov T, Kratz C, Knüchel R, Müller-Hermelink HK, Rosenwald A, Frey N, Eichler J, Dobrev D, El-Armouche A, Hengstler JG, Müller OJ, Hinrichs K, Cuello F, Zernecke A, Lorenz K 
In:
Nat Commun. (2020) 11: 1733 
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Primary Cells and Media 
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In:
Biochem Biophys Res Commun (2019) 514: 3 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kanwal A, Nizami HL, Mallapudi S, Putcha UK, Mohan GK, Banerjee SK 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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