Neural stem cell (NSC), mouse

Export

Stem cells derived from brain of mouse embryo and cultured as neurospheres.

Cell Type:
Neural SC
Tissue Origin:
brain
Species:
mouse
Research Area:
Neurobiology
Stem Cells
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
NSC, mouse A-033 4e6 35-45% Plasmid (general) 5 µg 100 µl I/II/2b
NSC, mouse A-033 4e6 50-70% Plasmid (general) 5 µg 100 µl I/II/2b
P3 DS-112 2e5 85% 80% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
P3 DS-113 2e5 88% 77% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations (26)

An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein.
Categories:
Primary Cells and Media, Transfection 
Authors:
Dewari PS, Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki M, Blin C, Finch R, Bressan RB, Morrison G, Jacobi AM, Behlke MA, von Kriegsheim A, Tomlinson S, Krijgsveld J, Pollard SM. 
In:
eLife (2018) 11;7: 1-29 
Efficient CRISPR/Cas9-assisted gene targeting enables rapid and precise genetic manipulation of mammalian neural stem cells.
Categories:
Transfection 
Authors:
Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S, Skarnes WC, Pollard SM. 
In:
Development (2017) 144(4): 635-648 
High-efficiency transfection and survival rates of embryonic and adult mouse neural stem cells achieved by electroporation.
Categories:
Transfection 
Authors:
Bertram B, Wiese S, von Holst A 
In:
J Neurosci Methods (2012) 209(2): 420-7 
Expression of GD2 and GD3 gangliosides in human embryonic neural stem cells
Categories:
Primary Cells and Media 
Authors:
Yanagisawa M, Yoshimura S, Yu RK 
In:
ACS Nano (2011) 3(2): e00054 
Meteorin promotes the formation of GFAP-positive glia via activation of the Jak-STAT3 pathway.
Categories:
Transfection 
Authors:
Lee HS, Han J, Lee SH, Park JA, Kim KW 
In:
J Cell Sci (2010) 123(Pt 11): 1959-68 
Histone demethylase LSD1 regulates neural stem cell proliferation.
Categories:
Transfection 
Authors:
Sun G, Alzayady K, Stewart R, Ye P, Yang S, Li W, Shi Y 
In:
Mol Cell Biol (2010) 30(8): 1997-2005 
A feedback loop comprising lin-28 and let-7 controls pre-let-7 maturation during neural stem-cell commitment
Categories:
Transfection 
Authors:
Rybak A, Fuchs H, Smirnova L, Brandt C, Pohl EE, Nitsch R, Wulczyn FG 
In:
Nat Cell Biol (2008) 10(8): 987-93 
Long-term transgene expression in mouse neural progenitor cells modified with PhiC31 integrase
Categories:
Transfection 
Authors:
Keravala A, Ormerod BK, Palmer TD, Calos MP 
In:
J Neurosci Methods (2008) 173(2): 299-305 
Epigenetic regulation of the stem cell mitogen FGF-2 by mbd1 in adult neural stem/progenitor cells
Categories:
Transfection 
Authors:
Li X, Barkho BZ, Luo Y, Smrt RD, Santistevan NJ, Liu C, Kuwabara T, Gage FH, Zhao X 
In:
J Biol Chem (2008) epub: online 
Ephrins as negative regulators of adult neurogenesis in diverse regions of the central nervous system
Categories:
Transfection 
Authors:
Jiao JW, Feldheim DA, Chen DF 
In:
Proc Natl Acad Sci USA (2008) 105(25): 8778-83 
The three SoxC proteins--Sox4, Sox11 and Sox12--exhibit overlapping expression patterns and molecular properties
Categories:
Transfection 
Authors:
Dy P, Penzo-Mendez A, Wang H, Pedraza CE, Macklin WB, Lefebvre V 
In:
Nucleic Acids Res (2008) 36(9): 3101-17 
Gli1 Induces G2/M Arrest and Apoptosis in Hippocampal but not Tumor-Derived Neural Stem Cells
Categories:
Transfection 
Authors:
Galvin KE, Ye H, Erstad DJ, Feddersen R, Wetmore C 
In:
Stem Cells (2008) 26(4): 1027-36 
BM88/CEND1 coordinates cell cycle exit and differentiation of neuronal precursors
Categories:
Transfection 
Authors:
Politis PK, Makri G, Thomaidou D, Geissen M, Rohrer H, Matsas R 
In:
Proc Natl Acad Sci USA (2007) 104(45): 17861-6 
Cyclosporin A inhibits mouse cytomegalovirus infection via a cyclophilin-dependent pathway specifically in neural stem/progenitor cells
Categories:
Transfection 
Authors:
Kawasaki H, Mocarski ES, Kosugi I, Tsutsui Y 
In:
J Virol (2007) 81(17): 9013-23 
A Cre-lox approach for transient transgene expression in neural precursor cells and long-term tracking of their progeny in vitro and in vivo
Categories:
Transfection 
Authors:
Geoffroy CG, Raineteau O 
In:
BMC Dev Biol (2007) 7: 45 
Neural stem/prognitor cells express 20 tenascin C isoforms that are differentially regulated by pax6
Categories:
Transfection 
Authors:
von Holst A, Egbers U, Prochiantz A, Faissner A 
In:
J Biol Chem (2007) 282(12): 9172-81 
The transcriptional coactivator querkopf controls adult neurogenesis
Categories:
Transfection 
Authors:
Merson TD, Dixon MP, Collin C, Rietze RL, Bartlett PF, Thomas T, Voss AK 
In:
J Neurosci (2006) 26(44): 11359-70 
Notch signalling regulates stem cell numbers in vitro and in vivo
Categories:
Transfection 
Authors:
Androutsellis-Theotokis A, Leker RR, Soldner F, Hoeppner DJ, Ravin R, Poser SW, Rueger MA, Bae SK, Kittappa R, McKay RD 
In:
Nature (2006) 442(7104): 823-826 
Dopachrome tautomerase (Dct) regulates neural progenitor cell proliferation
Categories:
Transfection 
Authors:
Jiao Z, Zhang ZG, Hornyak TJ, Hozeska A, Zhang RL, Wang Y, Wang L, Roberts C, Strickland FM, Chopp M 
In:
Dev Biol (2006) 296(2): 396-408 
Notch controls proliferation and differentiation of stem cells in a dose-dependent manner
Categories:
Transfection 
Authors:
Guentchev M, McKay RD 
In:
Eur J Neurosci (2006) 23(9): 2289-96 
Olig2 overexpression induces the in vitro differentiation of neural stem cells into mature oligodendrocytes
Categories:
Transfection 
Authors:
Copray S, Balasubramaniyan V, Levenga J, de Bruijn J, Liem R and Boddeke E 
In:
Stem Cells (2006) 24(4): 1001-1010 
Uncoupling and endocytosis of 5-hydroxytryptamine 4 receptors. Distinct molecular events with different GRK2 requirements
Categories:
Transfection 
Authors:
Barthet G, Gaven F, Framery B, Shinjo K, Nakamura T, Claeysen S, Bockaert J and Dumuis A 
In:
J Cell Biol (2005) 280(30): 27924-27934 
Electroporation-based gene transfer for efficient transfection of neural precursor cells
Categories:
Transfection 
Authors:
Richard I, Ader M, Sytnyk V, Dityatev A, Richard G, Schachner M and Bartsch U 
In:
Brain Res Mol Brain Res (2005) 138(2): 182-190 
Transient expression of olig1 initiates the differentiation of neural stem cells into oligodendrocyte progenitor cells
Categories:
Transfection 
Authors:
Balasubramaniyan V, Timmer N, Kust B, Boddeke E and Copray S 
In:
Stem Cells (2004) 22(6): 878-882 
Nuclear export of OLIG2 in neural stem cells is essential for ciliary neurotrophic factor-induced astrocyte differentiation
Categories:
Transfection 
Authors:
Setoguchi T and Kondo T 
In:
J Cell Biol (2004) 166(7): 963-968 
Sox1 acts through multiple independent pathways to promote neurogenesis
Categories:
Transfection 
Authors:
Kan L, Israsena N, Zhang Z, Hu M, Zhao LR, Jalali A, Sahni V, Kessler JA 
In:
Dev Biol (2004) 269(2): 580-94 
+ Show All

Privacy | Legal | About Lonza

© Lonza. All rights reserved.