Neural stem cell (NSC), rat

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Stem cells derived from brain of rat embryo and cultured as neurospheres or adherently on fibronectin-coated plates.

Cell Type:
Neural SC
Tissue Origin:
brain
Species:
rat
Research Area:
Neurobiology
Stem Cells
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
NSC, rat A-033 4e6 44-48% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (7)

Comparison of ectopic gene expression methods in rat neural stem cells.
Categories:
Transfection 
Authors:
Kim W, Kim JH, Kong SY, Park MH, Sohn UD, Kim HJ. 
In:
J Physiol (2013) 17(1): 23-30 
Nucleofection is the most efficient non-viral transfection method for neuronal stem cells derived from ventral mesencephali with no changes in cell composition or dopaminergic fate
Categories:
Transfection 
Authors:
Cesnulevicius K, Timmer M, Wesemann M, Thomas T, Barkhausen T, Grothe C 
In:
Stem Cells (2006) 24(12): 2776-91 
Tumor necrosis factor alpha triggers proliferation of adult neural stem cells via IKK/NF-kappaB signaling
Categories:
Transfection 
Authors:
Widera D, Mikenberg I, Elvers M, Kaltschmidt C, Kaltschmidt B 
In:
BMC Neurosci (2006) 7: 64 
Overexpression of SOCS3 inhibits astrogliogenesis and promotes maintenance of neural stem cells
Categories:
Transfection 
Authors:
Cao F, Hata R, Zhu P, Ma YJ, Tanaka J, Hanakawa Y, Hashimoto K, Niinobe M, Yoshikawa K, Sakanaka M 
In:
J Neurochem (2006) 98(2): 459-70 
Glucocorticoid hormones decrease proliferation of embryonic neural stem cells through ubiquitin-mediated degradation of cyclin D1
Categories:
Transfection 
Authors:
Sundberg M, Savola S, Hienola A, Korhonen L, Lindholm D 
In:
J Neurosci (2006) 26(20): 5402-10 
Somatic mosaicism in neuronal precursor cells mediated by L1 retrotransposition
Categories:
Transfection 
Authors:
Muotri AR, Chu VT, Marchetto MC, Deng W, Moran JV, Gage FH 
In:
Nature (2005) 435(7044): 903-10 
Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells
Categories:
Transfection 
Authors:
Hsieh J, Nakashima K, Kuwabara T, Mejia E, Gage FH 
In:
Proc Natl Acad Sci USA (2004) 101(47): 16659-16664 
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