P815
Export
mast cell; derived from mastocytoma
Cell Type:
Granulocyte
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
T
C-005
1e6
57-67%
89.4-95.4%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
CA-137
2e5
73.5-91.5%
90% ±2
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
CA-137
1e6
82.3-92.3%
87.5% ±4
Plasmid (general)
2 µg
100 µl
4D X-Unit
Citations
CD28-stimulated ERK2 phosphorylation is required for polarization of the microtubule organizing center and granules in YTS NK cells
Categories:
Transfection
Authors:
Chen X, Allan DS, Krzewski K, Ge B, Kopcow H, Strominger JL
In:
Proc Natl Acad Sci USA (2006) 103(27): 10346-51