Dorsal root gang. (DRG), chicken

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A collection of neurons associated with the processing of sensory information. Found within the sensory portion of the spinal nerve, just as the nerve exits the spinal foramina.

Cell Type:
Neurons (Ganglia)
Tissue Origin:
brain
Species:
chicken
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Neuron, chicken O-003 2e6 20-40% Plasmid (general) 3 µg 100 µl I/II/2b

Citations (9)

LIM-kinase and slingshot are critical for neurite extension
Categories:
Transfection 
Authors:
Endo M, Ohashi K, Mizuno K 
In:
J Biol Chem (2007) 282(18): 13692-702 
Raf kinase signaling functions in sensory neuron differentiation and axon growth in vivo
Categories:
Transfection 
Authors:
Zhong J, Li X, McNamee C, Chen AP, Baccarini M, Snider WD 
In:
Nat Neurosci (2007) 10(5): 598-607 
Cytoplasmic dynein and LIS1 are required for microtubule advance during growth cone remodeling and fast axonal outgrowth
Categories:
Transfection 
Authors:
Grabham PW, Seale GE, Bennecib M, Goldberg DJ, Vallee RB 
In:
J Neurosci (2007) 27(21): 5823-34 
Attractive axon guidance involves asymmetric membrane transport and exocytosis in the growth cone
Categories:
Transfection 
Authors:
Tojima T, Akiyama H, Itofusa R, Li Y, Katayama H, Miyawaki A, Kamiguchi H 
In:
Nat Neurosci (2007) 10(1): 58-66 
RhoA-kinase coordinates F-actin organization and myosin II activity during semaphorin-3A-induced axon retraction
Categories:
Transfection 
Authors:
Gallo G 
In:
J Cell Sci (2006) 119(Pt 16): 3413-23 
RhoA-kinase and myosin II are required for the maintenance of growth cone polarity and guidance by nerve growth factor
Categories:
Transfection 
Authors:
Loudon RP, Silver LD, Yee HF Jr, Gallo G 
In:
J Neurobiol (2006) 66(8): 847-67 
RanBPM contributes to Semaphorin3A signaling through plexin-A receptors
Categories:
Transfection 
Authors:
Togashi H, Schmidt EF, Strittmatter SM 
In:
J Neurosci (2006) 26(18): 4961-9 
PTEN couples Sema3A signalling to growth cone collapse
Categories:
Transfection 
Authors:
Chadborn NH, Ahmed AI, Holt MR, Prinjha R, Dunn GA, Jones GE, Eickholt BJ 
In:
J Cell Sci (2006) 119(Pt 5): 951-7 
Adaptation of sensory neurons to hyalectin and decorin proteoglycans
Categories:
Transfection 
Authors:
Lemons ML, Barua S, Abanto ML, Halfter W and Condic ML 
In:
J Neurosci (2005) 25(20): 4964-4973 
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