cells

Star-shaped glial cells that provide nutrients, support, and insulation for neurons of the central nervous system.

Are a mixed population isolated from the hippocampus, cortex, and striatum of the brain. These astrocytes are passaged once and cryopreserved.

Cell Type:

Glial Cells

Tissue Origin:

brain

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Neurobiology

Cell Characteristics:

Adherent

Recommended Media

Astrocyte Growth Medium

Astrocyte Growth Medium (AGM™) has been developed to support the growth of primary human astrocytes.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	Glial, basic	T-020	2e6	50-70%	60-70%	Plasmid (general)	5 µg	100 µl	I/II/2b

Citations

Efficient prime editing in mouse brain, liver and heart with dual AAVs

Categories:

Transfection

Authors:

Jessie R. Davis, Samagya Banskota, Jonathan M. Levy, Gregory A. Newby, Xiao Wang, Andrew V. Anzalone, Andrew T. Nelson, Peter J. Chen, Andrew D. Hennes, Meirui An, Heejin Roh, Peyton B. Randolph, Kiran Musunuru & David R. Liu

In:

Nature (2024) 42: 2

BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac module