721.221
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EBV transfected B cell line
Cell Type:
B cell
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
A-023
1e6
42%
53%
Plasmid (general)
µg
100 µl
I/II/2b
V
A-024
2e6
51%
71%
Plasmid (general)
2 µg
100 µl
I/II/2b
T
A-024
2e6
50%
70%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
WIP is essential for lytic granule polarization and NK cell cytotoxicity
Categories:
Transfection
Authors:
Krzewski K, Chen X, Strominger JL
In:
Proc Natl Acad Sci USA (2008) 105(7): 2568-73
NKG2D ligand expression in AML increases in response to HDAC inhibitor valproic acid and contributes to allorecognition by NK-cell lines with single KIR-HLA class I specificities.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Diermayr S, Himmelreich H, Durovic B, Mathys-Schneeberger A, Siegler U, Langenkamp U, Hofsteenge J, Gratwohl A, Tichelli A, Paluszewska M, Wiktor-Jedrzejczak W, Kalberer CP, Wodnar-Filipowicz A.
In:
Blood (2008) 111(3): 1428-36
CD8+ T cells from SIV elite controller macaques recognize Mamu-B*08-bound epitopes and select for widespread viral variation
Categories:
Transfection
Authors:
Loffredo JT, Friedrich TC, Leܳn EJ, Stephany JJ, Rodrigues DS, Spencer SP, Bean AT, Beal DR, Burwitz BJ, Rudersdorf RA, Wallace LT, Piaskowski SM, May GE, Sidney J, Gostick E, Wilson NA, Price DA, Kallas EG, Piontkivska H, Hughes AL, Sette A, Watkins DI
In:
PLoS ONE (2007) 2(11): e1152
Many NK cell receptors activate ERK2 and JNK1 to trigger microtubule organizing center and granule polarization and cytotoxicity
Categories:
Transfection
Authors:
Chen X, Trivedi PP, Ge B, Krzewski K, Strominger JL
In:
Proc Natl Acad Sci USA (2007) 104(15): 6329-34
A Single Polymorphism Disrupts the Killer Ig-Like Receptor 2DL2/2DL3 D1 Domain
Categories:
Transfection
Authors:
Vandenbussche CJ, Dakshanamurthy S, Posch PE, Hurley CK
In:
J Immunol (2006) 177(8): 5347-57
CD28-stimulated ERK2 phosphorylation is required for polarization of the microtubule organizing center and granules in YTS NK cells
Categories:
Transfection
Authors:
Chen X, Allan DS, Krzewski K, Ge B, Kopcow H, Strominger JL
In:
Proc Natl Acad Sci USA (2006) 103(27): 10346-51