HMC3

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Human microglia clone 3 is a cell line developped in 1995 through SV40-dependent immortalization of human embryonic microglial cells

Cell Type:
Glial Cells
Tissue Origin:
brain
Species:
human
Research Area:
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 CA-137 2e5 90.5% 69% Plasmid (general) 20 µl 4D X-Unit
P3 CM-137 2e5 89.9% 59% Plasmid (general) 20 µl 4D X-Unit
P3 DS-120 2e5 82.4% 89% Plasmid (general) 20 µl 4D X-Unit
SF CM-158 5e5 Plasmid (general) 20 µl 4D X-Unit

Citations

TREM2 variants that cause early dementia and increase Alzheimer's disease risk affect gene splicing
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Kostantin Kiianitsa, Maria E Lukes, Brian J Hayes, Julianna N Brutman, Paul N Valdmanis, Thomas D Bird, Wendy H Raskind, Olena Korvatska 
In:
Brain (2024) :  
Interrogation of human microglial phagocytosis by CRISPR genome editing
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Jason Cheng-Yu Chang, Cheng-You Wang, Steven Lin 
In:
Frontiers in Immunology (2023) :  

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