Vero

normal kidney

Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
monkey
Cell Characteristics:
Adherent

Recommended Media

ProVero™ 1 Serum-free Medium is a protein-free medium designed to support the growth of MDCK and vero cells. It contains HEPES and sodium bicarbonate buffer.
The absence of proteins and only very low levels of human recombinant insulin facilitate both downstream processing and regulatory compliance.

Some Vero cell strains require additional supplementation with 5.0 µg/L rhEGF for optimal vero cell growth.

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DN-100 1e6 89-93% 79% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF DN-100 2e5 92-94% 93% Plasmid (general) 400 ng 20 µl 4D X-Unit
V V-001 1e6 77-81% 96-98% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (16)

Categories:
Cell Lines and Primary Cancer Cells 
Authors:
Markus Hoffmann  1 , Hannah Kleine-Weber  2 , Simon Schroeder  3 , Nadine Krüger  4 , Tanja Herrler  5 , Sandra Erichsen  6 , Tobias S Schiergens  7 , Georg Herrler  8 , Nai-Huei Wu  8 , Andreas Nitsche  9 , Marcel A Müller  10 , Christian Drosten  3 , Stefan Pöhlmann  11              
In:
Cell (2020) 1: 1 
Categories:
 
Authors:
Roman Wölfel  1 , Victor M Corman  2 , Wolfgang Guggemos  3 , Michael Seilmaier  3 , Sabine Zange  1 , Marcel A Müller  2 , Daniela Niemeyer  2 , Terry C Jones  2   4 , Patrick Vollmar  1 , Camilla Rothe  5 , Michael Hoelscher  5 , Tobias Bleicker  2 , Sebastian Brünink  2 , Julia Schneider  2 , Rosina Ehmann  1 , Katrin Zwirglmaier  1 , Christian Drosten  6 , Clemens Wendtner  7              
In:
Nature (2020) 1: 1 
Categories:
Primary Cells and Media 
Authors:
Kolb AW, Lee K, Larsen I, Craven M, Brandt CR 
In:
PLoS Pathog (2016) 12(3): ePub 
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Mickaël Bouvet,‡§,1,2 Adrien Lugari,¶,1,3 Clara C. Posthuma, Jessika C. Zevenhoven,? Stéphanie Bernard,¶,4 Stéphane Betzi,¶ Isabelle Imbert,‡§ Bruno Canard,‡§ Jean-Claude Guillemot,‡§ Patrick Lécine,** Susanne Pfefferle,‡‡,5 Christian Drosten,‡‡ Eric J. Snijder,? Etienne Decroly,‡§,6,7 and Xavier Morelli¶,6,8 
In:
Biochemistry and Molecular Biology (2014) 289: 37 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Miao Q, Santamaria C, Bailey D, Genest J, Ward BJ, Ndao M 
In:
Am J Pathol (2014) 184(4): 976-84 
Categories:
Primary Cells and Media 
Authors:
Wahl-Jensen V, Kurz S, Feldmann F, Buehler LK, Kindrachuk J, DeFilippis V, da Silva Correia J, Früh K, Kuhn JH, Burton DR, Feldmann H. 
In:
Other (2011) 5(10): ePub 
Categories:
Electrophoreses and Analysis  
Authors:
Susanne Pfefferle, Verena Krähling, Vanessa Ditt, Klaus Grywna, Elke Mühlberger, Christian Drosten 
In:
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Categories:
Transfection 
Authors:
Konzack S, Thies E, Marx A, Mandelkow EM, Mandelkow E 
In:
J Neurosci (2007) 27(37): 9916-27 
Categories:
Transfection 
Authors:
Sereinig S, Stukova M, Zabolotnyh N, Ferko B, Kittel C, Romanova J, Vinogradova T, Katinger H, Kiselev O, Egorov A 
In:
Clin Vaccine Immunol (2006) 13(8): 898-904 
Categories:
Transfection 
Authors:
Plant EP, Dinman JD 
In:
RNA (2006) 12(4): 666-73 
Categories:
Transfection 
Authors:
Sbalzarini I, Mezzacasa A, Helenius A and Koumoutsakos P 
In:
Biophys J (2005) 89(3): 1482-1492 
Categories:
Transfection 
Authors:
Vonderheit A, Helenius A 
In:
PLoS Biol (2005) 3(7): e233 
Categories:
Transfection 
Authors:
Plant EP, Perez-Alvarado GC, Jacobs JL, Mukhopadhyay B, Hennig M, Dinman JD 
In:
PLoS Biol (2005) 3(6): e172 
Categories:
Transfection 
Authors:
Bing Ni  1 , Xinfu Shi, Yang Li, Wenda Gao, Xiliang Wang, Yuzhang Wu 
In:
(2005) 10: 4 
Categories:
Transfection 
Authors:
Kittel C, Sereinig S, Ferko B, Stasakova J, Romanova J, Wolkerstorfer A, Katinger H, Egorov A 
In:
Virology (2004) 324(1): 67-73 
Categories:
Serum-free and Speciality Media 
Authors:
Jorritsma-Smit A, van Zanten CJ, Schoemaker J, Meulenberg JJM, Touw DJ, Kosterink JGW, Nijman HW, Daemen T, Allersma DP. 
In:
Eur J Pharm Sci () 143: 105096 
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