cells

Specie of unicellular holozoan eukaryote. It has mix of fungal and animal features.

Cell Type:

Tissue Origin:

Species:

Research Area:

Molecular Biology

Cell Characteristics:

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	P3	EN-138	1e6			CRISPR/Cas		20 µl	4D X-Unit
	P3	EN-138	1e6			Plasmid (general)		20 µl	4D X-Unit

Citations

CRISPR-Cas9 genome editing in Corallochytrium limacisporum,a key species for understanding animal origins

Categories:

Protozoa

Authors:

Patricia S. Ara, Elena Casacuberta, Clausio scazzocchio, Iñaki Ruiz-Trillo, Sebastián R. Najile

In:

Open Biology (2025) 15: 7

Stable transfection in protist Corallochytrium limacisporum identifies novel cellular features among unicellular animals relatives

Categories:

Transfection, Protozoa

Authors:

Aleksandra Kozyczkowska, Sebastián R. Najle, Eduard Ocaña-Pallarès, Cristina Areste, Victoria Shabardina, Patricia S. Ara, Iñaki Ruiz-Trillo, Elena Casacuberta

In:

Curr Biol (2021) 31: