Breast cancer-on-chip for patient-specific efficacy and safety testing of CAR-T cells

3D Cell Culture , Serum-free and Speciality Media 

Tengku Ibrahim Maulana , Claudia Teufel, Madalena Cipriano , Julia Roosz , Lisa Lazarevski , Francijna E van den Hil , Lukas Scheller , Valeria Orlova , André Koch , Michael Hudecek , Miriam Alb , Peter Loskill  

Cell Stem Cell (2024) 31: 7 

Gene editing of hematopoietic stem cells restores T-cell response in familial hemophagocytic lymphohistiocytosis

Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 

Dettmer-Monaco V, Weißert K, Ammann S, Monaco G, Lei L, Gräßel L, Rhiel M, Rositzka J, Kaufmann MM, Geiger K, Andrieux G, Lao J, Thoulass G, Schell C, Boerries M, Illert AL, Cornu TI, Ehl S, Aichele P, Cathomen T 

J Allergy Clin Immunol (2023) S0091: 6749(23)00989-2 

Targeted T cell receptor gene editing provides predictable T cell product function for immunotherapy

Transfection, Cell Lines and Primary Cancer Cells 

Thomas R Müller , Sebastian Jarosch 1, Monika Hammel , Justin Leube , Simon Grassmann  , Bettina Bernard 1, Manuel Effenberger , Immanuel Andrä , M Zeeshan Chaudhry , Theresa Käuferle , Antje Malo , Luka Cicin-Sain , Peter Steinberger , Tobias Feuchtinger , Ulrike Protzer , Kathrin Schumann , Michael Neuenhahn , Kilian Schober , Dirk H Busch  

Cell Rep Med (2021) 2(8): 100374 

CRISPR-Mediated Non-Viral Site-Specific Gene Integration and Expression in T Cells: Protocol and Application for T-Cell Therapy

Transfection 

Zelda Odé , Jose Condori , Nicolas Peterson , Sheng Zhou , Giedre Krenciute  

Cancers (2020) 12(6): 1704 

Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency.

Primary Cells and Media, Serum-free and Speciality Media, Transfection 

Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A. 

Nat Biotechnol (2019) 1: 1 

Orthotopic replacement of T-cell receptor a- and ß-chains with preservation of near-physiological T-cell function.

Primary Cells and Media, Transfection 

Schober K, Müller TR, Gökmen F, Grassmann S, Effenberger M, Poltorak M, Stemberger C, Schumann K, Roth TL, Marson A, Busch DH. 

Nat Biomed Eng (2019) 10: 01