HAP-1

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HAP1 cells are a near-haploid cell line derived from the KBM-7 cell line. KBM-7 was found in a patient with chronic myeloid leukemia (CML)

Cell Type:
Leukemia
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE EN-138 2e5 90% 60-70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE DS-138 2e5 85% 60-70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Efficient C• G-to-G• C base editors developed using CRISPRi screens, target-library analysis, and machine learning
Categories:
Transfection 
Authors:
Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, Adamson B, Liu DR. 
In:
Nat Biotechnol (2021) 39(11): 1414-1425 
Optimized design parameters for CRISPR Cas9 and Cas12a homology-directed repair
Categories:
Transfection 
Authors:
Schubert MS, Thommandru B, Woodley J, Turk R, Yan S, Kurgan G, McNeill MS, Rettig GR. 
In:
Scientific Reports (2021) 11(1): 19482 
Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Categories:
Transfection 
Authors:
Komor AC, Zhao KT, Packer MS, Gaudelli NM, Waterbury AL, Koblan LW, Kim YB, Badran AH, Liu DR. 
In:
Sci Adv. (2017) 3(8): 1-9 
A p53-dependent response limits the viability of mammalian haploid cells.
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Olbrich T, Mayor-Ruiz C, Vega-Sendino M, Gomez C, Ortega S, Ruiz S, Fernandez-Capetillo O. 
In:
Proc Natl Acad Sci USA (2017) 114(35): 9367-9372 

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