beta cell, mouse transgenic for SV40 large T antigen; The cell line was derived from a pancreatic tumor (insulinoma) arising in a transgenic mouse

Cell Type:
Pancreas Epith.
Tissue Origin:
Research Area:
Cell Characteristics:

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DJ-102 1e5 66% 82% ±4 Plasmid (general) 0.6 µg 20 µl 4D X-Unit
SF DJ-102 2e6 77% 49% ±5 Plasmid (general) 2 µg 100 µl 4D X-Unit