D425

cells isolated after a biopsy of cerebellar primary tumor in 6- yr-old boy. medullablastoma cell line. growing in suspension but tend to aggregate.

Cell Type:
Neuroblast
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CA-137 3e5 70% 53% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF CA-137 3e5 siRNA 20 µl 4D X-Unit