cells

The Lund human mesencephalic (LUHMES) cell line was generated by conditional immortalization of a neuronal human precuros cells from ventral mesencephalic tissue of a female fetus. It is a subclone of the tetracycline-controlled, v-myc-overexpressing human mesencephalic-derived cell line MESC2.10. MESC2.10 was originally immortalized with a LINX v-myc retroviral vector.

LUHMES cells can be differentiated into morphologically and biochemically mature dopamine-like neurons following exposure to tetracycline, GDNF (glial cell line-derived neurotrophic factor), and db-cAMP. LUHMES cells exhibit the same dopaminergic and neuronal characteristics as MESC2.10 cells.

Cell Type:

Neurons (Brain)

Tissue Origin:

brain

Species:

human

Research Area:

Neurobiology

Cell Characteristics:

Suspension

Transfection Information

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Citations

A novel CRISPR/Cas9-based iduronate-2-sulfatase (IDS) knockout human neuronal cell line reveals earliest pathological changes

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Badenetti L, Manzoli R, Trevisan M, D'Avanzo F, Tomanin R, Moro E.

In:

Scientific Reports (2023) 13(1): 10289

Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders.

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Shah RR1, Cholewa-Waclaw J1, Davies FCJ2, Paton KM1, Chaligne R3, Heard E3, Abbott CM2, Bird AP1.

In:

BioResearch Open Access (2016) 13: 1