MIN6

pancreatic beta cell line, insulin secreting, insulinoma

Cell Type:
Pancreas
Tissue Origin:
pancreas
Species:
mouse
Research Area:
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V G-016 1e6 65% 40% Plasmid (general) 2 µg 100 µl I/II/2b
T G-020 7e6 70% 50% Plasmid (general) 2 µg 100 µl I/II/2b
T G-017 7e6 60% 60% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Andrali SS, Qian Q, Ozcan S 
In:
J Biol Chem (2007) 282(21): 15589-96 
Categories:
Transfection 
Authors:
Vanderford NL, Andrali SS, Ozcan S 
In:
J Biol Chem (2007) 282(3): 1577-1584 
Categories:
Transfection 
Authors:
Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P and Stoffel M 
In:
Nature (2004) 432: 226-230