MIN6
Export
pancreatic beta cell line, insulin secreting, insulinoma
Cell Type:
Pancreas
Tissue Origin:
pancreas
Species:
mouse
Research Area:
Gastroenterology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
G-016
1e6
65%
40%
Plasmid (general)
2 µg
100 µl
I/II/2b
T
G-020
7e6
70%
50%
Plasmid (general)
2 µg
100 µl
I/II/2b
T
G-017
7e6
60%
60%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Glucose mediates the translocation of NeuroD1 by O-linked glycosylation
Categories:
Transfection
Authors:
Andrali SS, Qian Q, Ozcan S
In:
J Biol Chem (2007) 282(21): 15589-96
Glucose induces MafA expression in pancreatic beta cell lines via the hexosamine biosynthetic pathway
Categories:
Transfection
Authors:
Vanderford NL, Andrali SS, Ozcan S
In:
J Biol Chem (2007) 282(3): 1577-1584
A pancreatic islet-specific microRNA regulates insulin secretion
Categories:
Transfection
Authors:
Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P and Stoffel M
In:
Nature (2004) 432: 226-230