Epithelial, intestinal, human (InEpC)

CloneticsTM InEpC\'s are truly primary cells representing both villi (enterocytes, goblet, and enteroendocrine cells) and crypts structures.  In combination with human intestinal myofibroblasts (InMyoFib), InEpC are able to form very tight cell monolayer, representing a unique in vitro system to model human intestinal homeostasis. Human intestinal epithelial cells (InEpC) are of pivotal importance in the digestion and absorption of nutrients and the protection of the organism from bacteria and toxins found in the intestine. The intestinal epithelium is a highly organized cellular system maintained in a dynamic steady state by cells in proliferation and differentiation, which die and exfoliate into the lumen. Intestinal cells are often difficult to isolate due to contamination issues and usually have to be cultured and used for assays immediately after isolation from tissue.

Cell Type:
GI Tract Epith.
Tissue Origin:
intestine
Species:
human
Research Area:
Gastroenterology
Cell Characteristics:
Adherent

Recommended Media

 SmGM-2 was developed for the maintenance and expansion of human smooth muscle cells. This medium contains 5% FBS.

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations

Categories:
Primary Cells and Media 
Authors:
Takahashi Y1, Sato S2, Kurashima Y3, Lai CY4, Otsu M4, Hayashi M5, Yamaguchi T5, Kiyono H6. 
In:
EBioMedicine (2017) 23: 34-45 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Carr RM, Qiao G, Qin J, Jayaraman S, Prabhakar BS, Maker AV 
In:
Cell Death Dis. (2016) 2: doi: 10.1038/cddiscovery.2016.67 
Categories:
Primary Cells and Media 
Authors:
Toshihiro Tadano, Yoichi Kakuta, Shin Hamada, Yosuke Shimodaira, Masatake Kuroha, Yoko Kawakami, Tomoya Kimura, Hisashi Shiga, Katsuya Endo, Atsushi Masamune, Seiichi Takahashi, Yoshitaka Kinouchi, and Tooru Shimosegawa 
In:
World J Gastroenterol (2016) 8(7): 532-542 
Categories:
Primary Cells and Media 
Authors:
Aleandri M, Conte MP, Simonetti G, Panella S, Celestino I, Checconi P, Marazzato M, Longhi C, Goldoni P, Nicoletti M, Barnich N, Palamara AT, Schippa S, Nencioni L. 
In:
PLoS ONE (2015) 10(2): e0117005